eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
1
16
10.21608/ejbo.2017.885.1065
3618
Ameliorative effect of Yeast Extract, IAA and Green-synthesized Nano Zinc Oxide on the Growth of Cu-stressed Vicia faba Seedlings
Wedad Kasim
wedkasim@yahoo.com
1
Essam AboKassem
e-abokassem@hotmail.com
2
Gehad Ragab
gehad_239@yahoo.com
3
Botany Department, Faculty of Science, Tanta University
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
Botany Department,Faculty of Science, Tanta University, Tanta, Egypt
T HE EFFECT of CuSO4 stress on some growth parameters and some metabolites in 14-day-old Vicia faba seedlings was studied. Irrigation with 150 mM CuSO4 resulted in remarkable reduction in root length, shoot height, fresh and dry weights of root and shoot, leaf area and photosynthetic pigments (Chl a, Chl b and carotenoids), while it raised Chl a/b ratio. Total soluble carbohydrates and protein contents were significantly depleted under Cu treatment. The ameliorative role of seed priming with different concentrations of yeast extract (25, 50, or 100 %), IAA (0.1, 0.3, 0.5 mM), or the green-synthesized Nano zinc oxide with average size 61 (nm) prepared from leaf extract of Coriandrum sativum (10, 50, 100 ppm) for different periods (6 and10h for yeast and IAA, 3 and 6h for Nano ZnO were studied. Results revealed that seed priming with 50% yeast or 0.1 mM IAA for 10h showed a remarkable increase in the measured growth criteria, chla, chl b, soluble carbohydrates and proteins but diminished chl a/b ratio compared with the Cu stress treatment. Seed priming with 100 ppm Nano ZnO for 6 h exhibited the most effective treatment in mitigating the harmful effects of Cu stress on growth criteria, Chla, Chl b, soluble carbohydrates and proteins.
https://ejbo.journals.ekb.eg/article_3618_08a95b5f13649755dada85a19b43ba56.pdf
copper
IAA
metabolites
Nano ZnO
Vicia faba
yeast
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
17
30
10.21608/ejbo.2017.944.1076
3619
Exopolysaccharides Production and Characterization from Marine-Derived Penicillium commune KP942881.1 with Some Medical Potential Applications
Alaa Abou zied
alaaabouzied@yahoo.com
1
Eman Abd El-Zaher
eimanabdelzaher@yahoo.com
2
Hassan Elsharquawy
drhassanelsharquawy@gmail.com
3
Toka Hammad
saad_toka@yahoo.com
4
Microbiology Department, Faculty of Science, Tanta University
Microbiology Department, Faculty of Science, Tanta University.
Microbiology Department, Marine Environment Division, National Institute of Oceanography and Fisheries (NIOF), Alexandria, Egypt
Microbiology Department, Faculty of Science, Tanta University, Egypt
SEVEN common marine-derived fungi were isolated from different collection sites distributed in Eastern and Western harbor of Alexandria, Egypt. The most promising marine-derived fungusproducing exopolysaccharide (EPS) was Penicillium commune (KP942881.1) which was identified according to microscopic morphological features and confirmed genetically by 18S rRNA gene. The results of the optimizing conditions for EPS production from marine-derived P. commune showed that 40 mg/ml of sucrose, 20 mg/ml of peptone, pH 5 and 3 cm discs of inoculum size and incubation at 30ºC, for 9 days were the optimal conditions with using static condition for all factors. Three main spectroscopic analyses (FTIR, 1H NMR and HPLC) were employed to characterize the EPS extracted from marine-derived P. commune.1HNMR analysis of EPS exhibited the presence of β-galatopyranosyl. The HPLC chromatography showed that the EPS consist of two peaks; raffinose and rhamnose. EPS showed antiproliferative activity at dose 10 mg/ml where the percentage inhibition of tumor viability cells of colon was 85%. In breast cell (Mcf-7), EPS inhibited 87% of the tumor cells at dose 10 mg/ml and also the antiviral activity of EPS of P. commune exhibited 22.8% inhibition.
https://ejbo.journals.ekb.eg/article_3619_9e1ba65711e19e928ae429e477e37144.pdf
Marine-Derived Penicillium commune
Exopolysaccharides
Characterization
Anti-tumor
Antiviral
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
31
43
10.21608/ejbo.2017.1017.1089
4085
Streptomyces violaceoruber ES: A Producer of Bioprospective Metabolite for Rapid and Green Synthesis of Antibacterial Silver Nanoparticles
Mohammed Abou-Dobara
aboudobara@gmail.com
1
Ahmed El-Sayed
akaelsayed@yahoo.co.uk
2
Noha Omar
noha_omar15@yahoo.com
3
Botany & Microbiology Department, Faculty of Science, Damietta University, Damietta El-Gededa, Egypt.
Botany & Microbiology Department, Faculty of Science, Damietta University, Damietta El-Gededa, Egypt.
Botany & Microbiology Department, Faculty of Science, Damietta University, Damietta El-Gededa, Egypt.
AN ISOLATE of Streptomyces was phenotypically, morphologically and physiologically characterized and identified as Streptomyces violaceoruber ES. Its identification was confirmed using 16S rRNA gene sequencing. It produced a metabolite containing deep pink pigment that might be involved in silver nanoparticles (AgNPs) biosynthesis. Both crude metabolite on starch nitrate medium and partially purified metabolite with ammonium sulphate precipitation were tested for AgNPs synthesis. The biosynthesis protocol had environmental advantages of green synthesis and superior nanoparticles properties included regular rounded shape, size range from 13 nm to 27 nm, and bactericidal efficiency. The AgNPs synthesis was performed within one minute at room temperature and sun light. This guarantees high biosafety, low cost, less consumption of energy, rapid and simplicity of its biosynthesis. Furthermore, the synthesized AgNPs showed antibacterial activity against multidrug resistant strains of E. coli, K. pneumoniae and P. aeruginosa. Also, it had synergistic effect with gentamycin against P. aeruginosa.
https://ejbo.journals.ekb.eg/article_4085_e881c599dd900623b8887122ac3862f0.pdf
Streptomyces
16S rDNA sequencing
silver nanoparticles
Antibacterial
Green synthesis
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
45
59
10.21608/ejbo.2017.831.1057
4086
Alleviation of Drought Stress in Vicia faba by Seed Priming with Ascorbic Acid or Extracts of Garlic and Carrot
Wedad Kasim
wedkasim@yahoo.com
1
Afaf Nessem
2
Azza Gaber
azzaeagle39@gmail.com
3
Botany Department, Faculty of Science, Tanta University
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.
THIS STUDY was carried out to assess the harmful effects of drought stress on Vicia faba L. (cv. Giza 716) and to evaluate the role of priming of the seeds in the extract of carrot root, garlic cloves or in ascorbic acid in the alleviation of these harmful effects. Fourteen days old Vicia faba seedlings grown from primed seeds were subjected to water withholding for 14 days (D1) or 22 days (D2), then left to grow till 40 days. The results revealed that the two levels of drought stress significantly decreased shoot height, fresh and dry masses and water content of root and shoot, as well as leaf area, but they increased root length of 40-days-old plants. Photosynthetic pigments (chl a and chl b), chlo a/chl b ratio, photosynthetic activity, sucrose and starch content were also decreased under the effect of both periods of drought stresses. On the other hand, carotenoid contents, the activities of catalase and peroxidase, ascorbic acid, malondialdehyde (MDA), glucose and total soluble protein contents were increased in leaves. Priming of Vicia faba seeds with the extracts of carrot root (Daucus carota L.), garlic cloves (Allium sativum L.) or with ascorbic acid mitigated all of the recorded harmful effects of drought stress,while they decreased carotenoids, activities of catalase and peroxidase, ascorbic acid, MDA, glucose and total soluble protein contents.
https://ejbo.journals.ekb.eg/article_4086_2f1c00090ddc43fba46991cb64f4c708.pdf
antioxidant
ascorbic acid
Carrot extract
drought
garlic extract
Osmolyte
Vicia faba
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
61
72
10.21608/ejbo.2017.926.1073
4135
Behavior of Esterase, Peroxidase, Protein Profile and Growth Parameters of Zea maize and Vicia faba Cultivar under Heat Stress
Doaa Mostafa
moustafadoaa@yahoo.com
1
Botany and Microbiology Department , Faculty of Science, Minia University.
IN THE present study, the effect of temperature (20/10 ºC and 35/20 ºC) on the activities of esterase, peroxidase, protein profile and growth parameters in two different plants (Vicia faba cv. Assuit 95/2 and Zea maize cv. Hi Tech 20*30) was investigated. Vicia faba cv. Assuit 95/2 was grown in pots at optimum temperature 20/10 ºC exposed to heat stress 35/20 ºC. While Zea maize cv. Hi Tech 20*30 was grown in pots at optimum temperature 35/20 ºC exposed to cooling stress 20/10ºC for 15 days. Behavior of esterase, peroxidase, protein profile, growth parameters, and metabolic constituents of Vicia faba and Zea maize plants varied between increase and shortage under heat stress and also between two organs shoot and root. Under high temperature, the growth parameters (fresh weights, dry weights, length and leaf area) were greatly reduced. It can be observed that the two plants responded to the unfavorable conditions (heat stress) by the change in their activities of enzymes, in the number of isoenzymes which include esterase (EST) and peroxidase (PX) and also changes in their biochemical composition actor in the amount of protein, carbohydrates and proline.
https://ejbo.journals.ekb.eg/article_4135_f3a89256061b3834a8aece0e4bea97c6.pdf
Heat stress, Protein profile, Isoenzymes
Proline, Vicia faba cv. Assuit 95/2, Zea maize cv. Hi Tech 20*30
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
73
83
10.21608/ejbo.2017.1088.1099
4136
Monitoring of Foodborne Hepatitis A virus Outbreaks in the Fresh Foods
Khaled El-dougdoug
drdougdoug@yahoo.com
1
Mohamed Nasr-Eldin
nasreldeen.m@gmail.com
2
Heba Esawy
heba.essawy@fsc.bu.edu.eg
3
Mahmoud Amer
mahmoud.amer@fsc.bu.edu.eg
4
Mai Abd Elrhiem
maiabdelrahem@yahoo.com
5
Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt
Botany Department, Faculty of Science, Benha University, Benha, Egypt.
Botany Department, Faculty of Science, Benha University, Benha, Egypt.
Botany Department, Faculty of Science, Benha University, Benha, Egypt
Botany Department, Faculty of Science, Benha University, Benha, Egypt
HEPATITIS A virus (HAV) is one of the most widespread foodborne pathogens and the cause of viral hepatitis. Fresh foods can be considered as a vector of transmission for HAV when contaminated by spoiled irrigation water or when prepared by infected food handlers. To improve microbiological detection and to increase insights into the contribution of fruit and vegetables to foodborne viral transmission, sensitive and standardized methods are needed. Two outlet drainage water samples from El-Mariotia and El-Gable Elasfar canals (Nile River) were collected in each month starting from March 2015 over a period of 12 months. As well as vegetables and fruit samples (lettuce, green onion and strawberry) were irrigated and washed with drainage water were also collected. Samples were extracted and concentrated for viral analysis. Strawberry and lettuce foods collected from El-Mariotia and lettuce from El-Gable Elasfar gave positive serological results by enzyme-linked immunosorbent assay (ELISA) test.Reverse transcription polymerase chain reaction (RT-PCR) was successfully used to detect the virus in strawberry and lettuce samples using HAV-specific primers, designed to amplify a 500 bp fragment covering VP1/2A gene in HAV. The VP1/2A gene was sequenced and the nucleotide sequence similarity was in the range of 95- 99.1% between HAV-Eg isolate and 30 HAV sequences retrieved from GenBank. Phylogenetic analysis revealed that HAV-Eg isolate was grouped into a clade comprising Egyptian HAV isolates sub-genotype IB. Phylogenetic tree of nucleotides sequence showed that HAV sub- genotype IB is the circulating strain.
https://ejbo.journals.ekb.eg/article_4136_5b853d6ed8c6f3971dfcf1b5c967c25f.pdf
hepatitis A virus
Fresh foods
Cell line culture
Serological detection
RT-PCR
Sub-genotype IB
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
85
102
10.21608/ejbo.2017.1070.1094
4137
Response of Salt-Stressed Wheat ( Triticum aestivum L.) to Potassium Humate Treatment and Potassium Silicate Foliar Application
Mohamed EH Osman
elanwar_osman@yahoo.com
1
Awatif Mohsen
awa_mohsen@yaho0.com
2
Soad Elfeky
sd_elfeky@yahoo.com
3
Walaa Mohamed
walaa_abd_elmegeid@yahoo.com
4
Botany department, Faculty of science, Tanta University, Egypt
Botany department, Faculty of science, Tanta University, Tanta, Egypt.
Botany Department, Faculty of Science, Tanta University
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt.
THE PRESENT study was carried out to investigate the effect of high NaCl concentrations on some growth parameters and physiological processes of wheat (Triticum aestivum L.) plant. The results indicated that Gemeza.9 was the most sensitive cultivar to high salinity levels compared to all tested wheat cultivars. Moreover, salinity stress caused a reduction in the germination percentage by 65% and all growth parameters by 21%, 25% and 60% in the lengths of shoot, root and leaf area, also by 27% and 48% in fresh weights of shoot, root and 40% and 75% in dry weights of shoot and root. It also increased the activity of antioxidant enzymes (peroxidase and catalase), lipid peroxidation and ascorbic acid content. Salinity induced increase of osmolytes compounds such as total soluble proteins, carbohydrates and amino acids. Furthermore, all measured yield parameters, the percentage of grain’s maturity and productivity were highly decreased. Accordingly, the role of potassium humate and potassium silicate either sole or combined in alleviating the toxic effect of salinity was also studied. Results showed that application of potassium humate as sole had a stimulatory effect higher than potassium silicate or their combination. It increased the germinating percentage by 128% at 200mM NaCl. In addition, potassium humate and potassium silicate increased the photosynthetic activity, decreased the activity antioxidant enzymes (Peroxidase and Catalase) as well as biosynthesis of MDA and ascorbic acid also they induced increase biosynthesis of proteins and carbohydrates in yielded grains. Accordingly, our study recommends the application of potassium humate as an organic fertilizer and potassium silicate as a foliar spray for improving the quality and quantity of the sensitive wheat cultivar Gemeza.9 cultivated in salty lands and increases its productivity.
https://ejbo.journals.ekb.eg/article_4137_f368a3c6ff9e2e548ad346b4ef345c81.pdf
salinity
amelioration
foliar application
potassium humate
potassium silicate
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
103
110
10.21608/ejbo.2017.837.1058
4138
Cytological Studies on Some Egyptian species of Anthemideae (Asteraceae)
Abd El-Tawab Atta
abdeltawab_ata@mu.edu.eg
1
Magdy Abd El-Tawab
magdyh2@yahoo.com
2
Rasha Helmey
rashahelmey@yahoo.com
3
Hadeer Dahy
hadeer_dahy@yahoo.com
4
Genetics Department, Faculty of Agriculture, Minia University, Egypt
Botany and Microbiology Department, Faculty of Science, Minia University, Egypt.
Botany & Microbiology Department-Faculty of Science-Minia University
Botany and Microbiology Department, Faculty of Science, Minia University, Egypt.
THE MEIOTIC behavior and morphology of pollen grains of four species of Asteraceae (Glebionis coronaria, Anacyclus monanthos, Matricaria recutita and cotula barbata) were studied. Glebionis coronaria, Anacyclus monanthos and Matricaria recutita showed nine chromosome bivalents while those of Cotula barbata showed eight chromosome bivalents. The chromosomes were meiotically identified as two pairs in three taxa (Glebionis coronaria, Anacyclus monanthos and Matricaria recutita) while it was one pair in Cotulabarbata.The pollen shape and morphology were recognized by measuring polar and equatorial axis, Morphological characteristics of the pollen grains of the four studied species were considerably different.
https://ejbo.journals.ekb.eg/article_4138_7265a1823d7283d69ba7e7d694502b35.pdf
Cytological studies
Egyptian species of Anthemideae
Meiotic
Pollen morphology
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
111
118
10.21608/ejbo.2017.980.1087
4146
First Record of Lepista sordida (Schumach) Singer in Eastern North Africa
Amira El_Fallal
aelfallal55@gmail.com
1
Ahmed Elsayed
akaelsayed@yahoo.com
2
Hoda El_Gharabawy
hoda_mohamed@du.edu.eg
3
Botany and Microbiology Department, Faculty of Sciences, Damietta University
Botany and Microbiology Department, Faculty of Sciences, Damietta University
Botany and Microbiology Department, Faculty of Science, Damietta University
A NEW record of Lepista sordida was collected from a lemon fruit farm in El-Sinania at Damietta District of North delta of Egypt in December, 2014. It was identified using morphological (macro and microscopic) and molecular techniques. Complete description was preformed for the collected fresh fruiting bodies and isolated pure culture. Radial growth rate of culture was estimated on Potato dextrose and Malt extract agar media (8.5 & 7.8 mm/ day, respectively). Lepista sordida and L. nuda are very close in morphological characters; hence, the identification was confirmed by DNA sequence analysis of the ribosomal 5.8S rRNA gene including the flanking internal transcribed spacers (ITS). Then, its taxonomic position among some genera of Tricholomataceae and its relation with some other Lepista species was discussed. The surrounding habitat was also observed and environmental conditions were recorded as Temperature degree (29.7oC), relative humidity (RH: 44.6) and soil moisture was (5.56). Lepista sordida was reported from South Africa and Nigeria (in the South), Algeria and Tunisia (in the North-west) and this is first record in North-East Africa.
https://ejbo.journals.ekb.eg/article_4146_76c9c5581505cb7aaa755abde73a9bc2.pdf
Tricholomataceae
Lepista sp
Edible mushroom
morphological identification
phylogenetic tree
ITS DNA sequencing
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
119
132
10.21608/ejbo.2017.1032.1091
4175
Short Term Effect of He-Ne Laser on Jatropha curcas Leaf Development and Amino Acid Biosynthesis: More Insights in Developmental Genetics of Oil Producing Plants
Noha Khalifa
nohakhalifa@hotmail.com
1
Hatem El-Ghandoor
hatemelghandoor@yahoo.com
2
Botany Dept., Faculty of Science, Ain Shams University, Cairo, Egypt
physics Dept, Faculty of Science, Ain Shams University
JATROPHA curcas is a promising plant for biodiesel production. One tentative way to increase oil production is using non traditional physical methods such as laser. It had been shown before that laser could enhance plant growth and development as general. In this study, the DNA was extracted from leaves of plants grown from seeds collected from different regions in Egypt and tested using PCR to confirm their homogenousity. Then, the fully expanded leaves of Jatropha (6- week- old plants) were exposed to a dose equaling 300 mJ cm-2 Helium-Neon (He-Ne) laser. The effect of the administered dose on the photosynthetic pigment expression, chloroplast biogenesis and total amino acid biosynthesis were evaluated after 0, 1 and 24 h post irradiation. Laser treatment in this work did enhance all the previousely mentioned parametrs as will be discussed further in relation to the possibility of using laser as a novel protocol to enhance field crop plants as general.
https://ejbo.journals.ekb.eg/article_4175_b2f2c52dc7df780dd6217a94ea892fb5.pdf
Laser
RAPD PCR
Chloroplast biogenesis
amino acid
Developmental genetics in oil plants
Jatropha curcas
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
133
145
10.21608/ejbo.2017.811.1051
4174
Seasonal Variations in Soil and Bacteriological Properties as Affected by Phytogenic Mounds (Nebkhas) of Halocnemum strobilaceum in Lake Manzala, Egypt
Heba Issa
hebahamed21@yahoo.com
1
Magdy El-Bana
mag_bana@yahoo.co.uk
2
Magdy Bahgat
magdybahgat@hotmail.com
3
Mohamed Dobara
aboudobara@du.edu.eg
4
Department of Botany, Faculty of Science, Port Said University, Egypt
Department of Botany, Faculty of Science, Port Said University
Department of Botany, Faculty of Science, Port Said University, Egypt
Department of Botany & Microbiology, Faculty of Science, Damietta University, Egypt
P HYTOGENIC mounds (nebkhas) formed by halophytes are widely distributed in coastal and desert environments. Such nebkhas provide loci for geochemical and biological activities that influence ecosystem processes and function. Seasonal variation in soil and bacterial properties were investigated within the nebkhas under the canopy of Halocnemum strobilaceum and in the surrounding unvegetated sites at salt marshes of Lake Manzala. The parameters measured include soil moisture, organic matter, total nitrogen and phosphorus, pH, electrical conductivity (EC), and bacterial community and enzyme characteristics. Enrichment ratios for soil salinity and nutrients were evaluated to indicate the salinity and fertility islands within nebkhas. Soil moisture content showed significant differences between the different seasons with the highest value in winter (16.55%) at unvegetated control site.Soil electrical conductivity, total nitrogen and phosphorus had their significantly higher values in nebkhas during autumn. The enrichment values for the measured soil parameters ranged from 0.31 to 4.09 in the four seasons which indicates a clear accumulation effect for soil nutrients and salts within the nebkhas under the canopy of H. strobilaceum. Bacterial abundance was consistently higher in nebkhas through all seasons. Eleven genera of bacteria belong to Firmicutes, Actinobacteria and proteobacteria phyla were recorded in nebkha and unvegetated sites. These genera varied in their occurrence between the two locations and the four seasons. It can be concluded that the formation of nebkhas by halophytic vegetation have noticeable effects on accumulation of soil salinity and fertility during different seasons that affect bacterial abundance and activity in coastal environment.
https://ejbo.journals.ekb.eg/article_4174_5840843692f7ae9b1f2e63f5d94c784b.pdf
Halocnemum strobilaceum
Nebkha
amylase
Carboxymethylcellulase
Bacteria
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
147
159
10.21608/ejbo.2017.847.1061
4176
Genetic Diversity of the Endemic Species Phlomis aurea Decne. in Southern Sinai, Egypt
Laila EL-Sadek
1
Ghada El-Badan
ghadaelbadan@hotmail.com
2
Hatem Shabana
3
Botany & Microbiology Department, Faculty of Science, Alexandria University, Alexandria
Botany & Microbiology Department, Faculty of Science, Alexandria University, Alexandria
Nature Conservation Sector, Egyptian Environmental Affairs Agency, Cairo, Egypt
PHLOMIS AUREA Decne. (Lamiaceae), which have future antidiabetic drug yielding potentials, is an endemic species, restricted to the high altitudes in five main habitats in southern Sinai. The genetic characterization of individuals from different populations is necessary to construct proper conservation programs. Thus, the present study was conducted to achieve two main goals; 1) Finding the genetic diversity among Phlomis aurea populations through variation in seed storage proteins electrophoretic pattern and Inter Simple Sequence Repeats (ISSR) finger printing as a dominant DNA molecular marker. 2) Perceive the relationship among these biochemical and molecular parameters with the different habitats in Saint Katherine Protectorate (SKP) are in South Sinai. Mature dry seeds from 30 individuals representing different populations were used for protein analysis using SDS-PAGE technique, 12 individuals of them were chosen for ISSR analysis. The former test produced 20 protein bands, three of them were common to all plants (species specific) and could be used as finger prints for Phlomis aurea. It was suggested that seed storage protein patterns are affected by aspect direction, altitude and slightly affected by habitats. ISSR analysis showed that decreasing genetic variation of Phlomis aurea individuals from different habitats in the following order: Wadibed> Slope> Gorge>Basin. This open area habitat contributes to high percent of hybridization and consequently results in high genetic polymorphism.
https://ejbo.journals.ekb.eg/article_4176_ea3054531e10455657d6faeb8d10dc64.pdf
Phlomis aurea
Seed storage protein
ISSR markers
Genetic diversity
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
161
172
10.21608/ejbo.2017.758.1044
4177
Antimicrobial Activity of Different Essential Oils against Aspergillus flavus and Klebsiella pneumoniae Isolated from Respiratory Tract
Wagih El-Shouny
elshounyw@yahoo.com
1
Eman Abd El-Zaher
eimanabdelzaher@yahoo.com
2
Samya Shabana
olaa_abdelsalam@yahoo.com
3
Olaa Mohammed
modykhaled57@yahoo.com
4
Microbiology Department, Faculty of Science, Tanta University, Tanta, Egypt.
Microbiology Department, Faculty of Science, Tanta University
Microbiology Department, Faculty of Science, Tanta University.
Microbiology Department, Faculty of Science, Tanta University.
ESSENTIAL oils from Cinnamomum verum (Cinnamon), Lavandula angustifoli (Lavender), Lepidium virginicum (Peppergrass oil), Zingiber officinale (Ginger) and Cinnamomum camphora (Camphor) have been investigated for their antifungal and antibacterial activities against 6 fungal isolates of Aspergillus flavus and 25 bacterial isolates of Klebsiella pneumoniae. The sensitivity was determined using agar well diffusion method and the inhibition zones were compared with the standard drug itraconazole for fungi and Amikacine for tested bacteria. The tested essential oil showed a wide spectrum of inhibition against the tested isolates. Treating the Klebsiella pneumoniae, with cinnamon essential oil led to an external changes, irregular cell shape and disintegration of bacterial cell wall under transmission electron microscope. GC-MS technique was used for cinnamon essential oil; the composition of cinnamon oil was dominated by cinnamaldehyde. The in vivo efficacy of cinnamon essential oil in treatment of Klebsiella pneumoniae in mice lungs was proven.
https://ejbo.journals.ekb.eg/article_4177_37dbaff5c397306f94f5efb669cc07eb.pdf
Essential oils
Antifungal
Antibacterial
GC-MS
TEM
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
173
185
10.21608/ejbo.2017.903.1067
4207
In vitro Antagonistic Activity of Trichoderma harzianum against Rhizoctonia solani The Causative Agent of Potato Black Scurf and Stem Canker
Mohsen Ibrahim
mohsenhbrahim@yahoo.com
1
Botany Department - Faculty of Science - Port Said University
IN EGYPT Potato (Solanum tuberosum L.) is considered one of the most important vegetable crops as well as many others countries in the world. It plays an important role in the Egyptian agricultural economy, not for local consumption but also for exportation especially to Europe, Russia and Arabic countries. However, potato crop suffer from more than 40 pests and diseases caused by insects, nematodes, viruses, bacteria and fungi, of which black scurf and stem canker induced by Rhizoctonia solani is probably the most serious disease. The world yield losses caused by R. solani were estimated to 5-15%. R. solani is an unspecialized parasite, survive in soil in the absence of host plant and make itself a very difficult pathogen to manage. Reduction or elimination of soil borne inoculum is the only effective solution to overcome the problem and this may be achieved through the application of various control measures of which fungal antagonists consider among the most important tactic. Although R. solaniis usually controlled through the application of chemicals, the serious ecological and financial toll of this fungus has prompted for research on biopesticides as a viable alternative. Trichoderma spp. were well-known fungi often used for the biological control of crop pests, whose anti-fungal mechanisms include competition for the substrate, antibiosis and/or mycoparasitism. Five isolates of Trichoderma harzianum were tested in vitro for their antagonistic potential against Rhizoctonia solani. Both Trichoderma harzianum and Rhizoctonia solani were identified by molecular and morphological methods. In dual culture of all isolates were found antagonistic to the growth of R. solani. The hyphal interaction studied using light microscopy revealed destructive mycoparasitism of R. solani by T. harzianum. The method of mycoparasitism was sparse to intense coiling of R. solani followed by disintegration, disorganization and death of R. solani mycelium.
https://ejbo.journals.ekb.eg/article_4207_26cabe1541c95de2c8581261330b8647.pdf
potato
black scurf
Rhizoctonia solani
Biocontrol
Trichoderma spp
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
187
196
10.21608/ejbo.2017.965.1080
4210
Epidemiology, Causative Agents and Clinical Features of Onychomycosis in El-Gharbia Governorate
Mohamed Bedaiwy
mbedaiwy@hotmail.com
1
Metwally Metwally
met1959@hotmail.com
2
Nessma Elzawawy
dr.nessmaelzawawy@yahoo.com
3
Heba Allah Saba
habahebo1983@yahoo.com
4
Botany department, Faculty of Science, Tanta university, Tanta, Egypt
Botany department, Faculty of Science, Tanta University, Tanta, Egypt
Botany department,faculty of science, Tanta university, Tanta, Egypt
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
ONYCHOMYCOSIS is a fungal disease of the nails that infects the nail plate, nail bed and matrix, caused by various species of dermatophytes, yeasts and molds. The aim of this investigation is to determine the clinicomycological and epidemiological profile of the etiologic agents of onychomycosis. The study population included 100 suspected cases of onychomycosis referred to the outpatient clinic at the Department of Dermatology at Tanta University Hospital, El-Gharbia Governorate, Egypt during 2015-2016. Nail clippings were collected in sterile Petri dishes for direct microscopic examination and culture. Fungal isolates were identified. The results showed that candidal onychomychosis was the most common clinical type. Candida tropicalis was the most prevalent causative species. It was more common among females aged 41–50 years. The incidence was higher during summer and autumn seasons. Housewives were the commonest infected population. Fingernails were affected more frequently than toenails. The pattern of causative agents and clinical signs of onychomycosis is altering region to region, so repeated epidemiological surveys of onychomycosis seems to be fundamental. The present study provided novel and appropriate epidemiologic data of onychomycosis for the better prevention and treatment of this fungal infection.
https://ejbo.journals.ekb.eg/article_4210_0507d6874f65730ee9ccaec231b1d9d8.pdf
Onychomycosis
Dermatophytes
Candida
Epidemiology
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
197
204
10.21608/ejbo.2017.971.1082
4254
The Immunoprotective Efficacy of Exopolysaccharides Produced from Different Strains of Pseudomonas syringae against Human Pathogenic Pseudomonas aeruginosa
Wagih El-Shouny
elshounyw@yahoo.com
1
Mohamed EH Osman
elanwar_osman@yahoo.com
2
Ragdah Talat
ragdahtalat@yahoo.com
3
Heba El-Zahaby
olaa_abdelsalam@yahoo.co
4
microbiology, science, tanta univercity
Tanta University, Egypt
microbiology, medecine, tanta university
microbiology, science, tanta univercity
EXOPOLYSACCHARIDES (EPS) produced from Pseudomonas syringae pv. tomato, P. syringae davson 973 and P. syringae pv. coriandricola 908 were studied. Mice immunized (i.p.) with bacterial EPS preparations from three strains (divided into 3 days) and in the fifth day, challenged (s.c.) with 1 ml of human pathogen P. aeruginosa 3 to each mouse, all groups of mice showed an increase in total leukocyte counts as well as neutrophil and monocyte numbers if compared with saline control group. Studing the active immunization by purified bacterial EPS on the counts of P. aeruginosa in lungs, spleens and livers of challenged mice revealed the protection of organs against bacteremia. Thus, the immunoprotective characters of the three purified EPSs could be concluded and recommended for prophylactic applications.
https://ejbo.journals.ekb.eg/article_4254_74076bf867020fb306b401758a1f2e31.pdf
Exopolysaccharides
P. aeruginosa
Mice
Immunization
Bacteremia
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
205
214
10.21608/ejbo.2017.912.1072
4381
Impact of The Microbial Suppression by Using The Brown Alga Dictyota dichotoma Extract
Ibraheem Ibraheem
ibraheemborie@gmail.com
1
Neveen Abdel-Raouf
neveenabdelraouf@science.bsu.edu.eg
2
Hanaa Mohamed
marawanahmed@hotmail.com
3
Rehab Fassihy
rehab.yahia55@gmail.com
4
Seham Hamed
seham_moussa939@yahoo.com
5
Botany and Microbiology department Faculty of Science,Beni-Suef University,Egypt
Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
Zoology Department Faculty of Science,Beni-Suef University, Egypt
Botany department,Faculty of Science, El-Minia University,Egypt
Soil Microbiology Department, Soils, Water and Environment Institute, Agricultural Research Center, Giza, Egypt.
IN THIS CONTEXT the brown alga Dictyota dichotoma isolated from Hurghada Sea-Shore, Red-Sea coast, Egypt (June 2015) was screened for its antimicrobial bioactivity. A laboratory experiment was conducted to test activity of ethanol extract of the brown alga Dictyota dichotoma against three types of Gram- positive and six of Gram-negative bacteria, and one type of fungi. Ethanol extract was selected for this study by using different concentrations (100, 200, 300, 400 mg) of alga extracts. Result revealed that the alga ethanolic extract exhibited high suppression activity against all tested pathogens. HPLC analysis of the chemical composition of algal extract was detect the presence of high amount of certain ingredients such as Flavonoids (Acacetin, Luteo.6-arabinose8-glucose, Naringin, Hespirtin, Rosmarinic and Naringenin by 3448.407, 453.209, 396.107, 387.429, 213.689 and 207.17 ppm, respectively) and Phenols (Benzoic, E-Vanillic, Pyrogallol, Catechein, Salycilic and Ellagic by 1075, 912, 785, 628 and 357 ppm, respectively). The present finding revealed that the tested alga can be used as possible natural source of active ingredients.
https://ejbo.journals.ekb.eg/article_4381_ab765390ad985dd6716aee2dceea906f.pdf
Dictyota dichotoma
antimicrobial activity
HPLC analysis
eng
National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)
Egyptian Journal of Botany
0375-9237
2357-0350
2017-10-01
57
7th International Conf.
215
232
10.21608/ejbo.2018.978.1085
4946
Study of the Interactive Effects of Calcium and Abscisic Acid on Drought Stressed Triticum aestivum Seedlings
Nasser Sewelam
sewelam@science.tanta.edu.eg
1
Botany Department, Faculty of Science, Tanta University, Tanta, Egypt
IN THEIR natural habitats, plants are continuously subjected to many severe environmental challenges including drought. In this study, the effects of drought on Triticum aestivum seedlings were assessed, and the alleviating roles and interactive effects of calcium (Ca2+) and abscisic acid (ABA) to drought stress were studied. The results showed that pretreating wheat seedlings with calcium chloride (CaCl2) or ABA led to alleviating most of the negative effects of 20% polyethylene glycol (PEG, as a drought imposing agent). Ca2+ improved the growth criteria, photosynthetic activity, but ABA had no significant effects on growth parameters. Ca2+ or ABA pretreatments have alleviated the toxic effects of the drought-induced oxidative stress which was marked with the reduction of MDA content. Ca2+ and/or ABA reduced the PEG-induced catalase activity, and ascorbic acid content. The use of lanthanum chloride (LaCl3) as a calcium channel blocker has confirmed the role of Ca2+ in ameliorating the drought effects on plant. This study revealed that the application of Ca2+ has increased the endogenous ABA level of the treated seedlings to a high extent exceeding that of PEG treatment itself suggesting that ABA requires Ca2+ ions for its induction, and ABA is working downstream from Ca2+ in the relevant signaling pathways activated by drought stress. The alleviating effects of Ca2+ was prolonged to the yield stage. Overall, our results suggest that Ca2+ (10 mM), and with a less extend ABA (0.1 mM), represent signaling agents that can partially alleviate drought stress in plants growing in dry environments.
https://ejbo.journals.ekb.eg/article_4946_18d1d37ea12daca370780f4d7ddf61eb.pdf
drought
polyethylene glycol
Calcium signaling
abscisic acid
Lanthanum chloride
antioxidants