National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Bryophytes of Libya. I. Hepatophyta: an Annotated Checklist277279358010.21608/ejbo.2017.545.1021ENSaid YoussefBotany Department, Faculty of Science, BenhaUniversity, Benha, EgyptJournal Article20170110THE list of Hepatophyta in Libya includes 29 taxa in 15 genera, 14 families, 7 orders and two classes. Anthocerotae is represented by one species while Hepaticae by 27 species. No species were reported after 1968; recent collections are therefore necessary. Riccia is dominant being represented by 12 species. Synonyms are given in a separate list.https://ejbo.journals.ekb.eg/article_3580_4e652ccb1362c536f4f1680f393b22f3.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Using Moringa olifera Leaf Extract as a Bio-fertilizer for Drought Stress Mitigation of Glycine max L. Plants281292329810.21608/ejbo.2017.596.1027ENRania HanafyBiological and Geological Sciences Department, Faculty of Education, Ain Shams University, Cairo, EgyptJournal Article20170124DROUGHT is one of a considerable environmental stresses limiting productivity of crops. This work was conducted to investigate the effect of foliar spray of plant leaf extract (Moringa olifera) to alleviate drought stress in Glycine maxplants (cv.Giza 111). Drought caused significant decreases in growth parameters (shoot and root length, fresh and dry weight of shoots and roots) and photosynthetic pigments (chlorophyll a, chlorophyll b, carotenoids and total pigments). In addition, it caused significant increasesi n non-enzymatic antioxidants (ascorbic acid, tocopherol and reduced glutathione), enzymatic antioxidants (glutathione reductase, superoxide dismutase and ascrobate peroxidase), oxidative damage (lipid peroxidation) and osmolyte compounds (proline, total soluble sugars and total phenols) in soybean plants. Moreover, foliar spray with Moringa leaf extract (MLE(30)) enhanced all the above parameters as compared with either the control plants and drought stressed plants. It appeared that MLE (30) was able to enhance the tolerance of the studied plant to drought stress.https://ejbo.journals.ekb.eg/article_3298_cede06b89dccd85078f56f872a6aae6f.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Glutathione Induced Antioxidant Protection Against Salinity Stress in Chickpea (Cicer arietinum L.) Plant293302361210.21608/ejbo.2017.636.1029ENMervat SadakBotany Department, Agricultural and Biological Division, National Research Centre, Giza, EgyptEbtihal Abd ElhamidBotany Department, Faculty of Science, Assiut University, New Valley Branch, EgyptMarwa MahmoudBotany Department, Faculty of Science, Assiut University, New Valley Branch, EgyptJournal Article20170204A POT experiment was conducted to evaluate the possible role of foliar treatment of glutathione in enhancing the antioxidant defense system of chickpea plant. Different concentrations (0, 50, 100 and 150 mg/L) of glutathione were used; namely GHS0, GHS1, GHS2 & GHS3, respectively under different levels of seawater salinity (0.23, 3.13 and 6.25 dS/m namely S0, S1 & S2, respectively). Seawater levels, S1& S2 caused obvious increases in proline, free amino acids and total soluble sugar contents. Moreover, foliar application of glutathione caused more significant increases in the above mentioned osmoprotectant contents of chickpea plants, as compared with those of corresponding controls. In addition, irrigation of chickpea plant with different levels of diluted seawater significantly increased hydrogen peroxide and lipid peroxidation, as compared with the tap water irrigated plants. Meanwhile, treating chickpea plants with different concentrations of glutathione resulted in significant decreases in hydrogen peroxide contents and lipid peroxidation levels in the control and salinity stressed plants. The applied concentrations of glutathione also resulted in marked increases of the antioxidant enzymes ascorbate peroxidase, glutathione reductase, peroxidase and superoxide dismutase. Maximum increases in the activities of antioxidant enzymes were observed on treating plants with glutathione at 100 mg/L either under normal irrigation or salinity stressed conditions. It could be concluded that foliar spray of glutathione was effective in improving chickpea performance by reducing hydrogen peroxide free radical and by enhancing compatible osmolytes, membrane stability and antioxidant enzyme activities.https://ejbo.journals.ekb.eg/article_3612_fe45271c9a17e957795cc6a9c1be31f0.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Occurrence of Atrazine Biodegrading Bacterium "Ochrobactrum oryzae" In Agricultural Wastewater303316370310.21608/ejbo.2017.647.1032ENAmany M.M.ReyadDepartment of Botany, Faculty of Science, Fayoum University, Fayoum, EgyptTharwat RadwanDepartment of Botany, Faculty of Science, Fayoum University,Fayoum, EgyptW. M.IbrahimBotany & Microbiology Department, Faculty of Science, Damanhour University, Damanhour, EgyptA. M.M.EssaDepartment of Botany, Faculty of Science, Fayoum University,Fayoum, EgyptJournal Article20170206ATRAZINE is the most extensively used herbicide that restrains photosynthesis of broad leaf and grassy weeds. In consequence of ground and surface water contamination with atrazine, humans and wildlife might be at risk. In the current investigation, an atrazine-degrading bacterium was isolated from agricultural drainage ditches (Fayoum, Egypt) using enrichment technique. According to the morphological, biochemical and 16S rDNA gene sequencing, the bacterial isolate was identified as Ochrobactrum oryzae. Using atrazine as sole carbon and nitrogen source, a pure culture of O. oryzae was grown in minimum media. The impact of atrazine concentration as well as temperature and pH on the bacterial growth and atrazine degradation rate was studied. The greatest potential for atrazine degradation (83.5%) was attained at concentration 400 ppm of atrazine within 9 days at pH value 9.0 and temperature 30°C. Consequently, O. oryzae can be applied conveniently for the cleanup of agricultural wastewater contaminated with high levels of atrazine.https://ejbo.journals.ekb.eg/article_3703_72bc5d329ac1e6c8de88902cf33fba59.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Physiological Studies on the Interactive Effects of Lead and Antioxidants on Carum carvi Plant317333361310.21608/ejbo.2017.653.1034ENR. AliBotany Department, Faculty of Science, Fayoum University, Fayoum, EgyptM. H.MahmoudBotany Department, Faculty of Science, Fayoum University, Fayoum, EgyptHesham AbbasBotany Department, Faculty of Science, Fayoum University, Fayoum, EgyptMarwa FakhrBotany Department, Faculty of Science, Fayoum University, Fayoum, EgyptJournal Article20170208AN EXPERIMENT was conducted to assess the responses of Carum carvi to lead stress and using ascorbic acid and α tochopherol as antioxidants. In this experiment, plants were grown in the nutrient medium with different levels of lead (0, 50, 100,200,500 and 1000 mM) in the form of lead nitrate Pb(NO3)2.Ascorbic acid or α tochopherol level (each 50 ppm) was used as seed presoaked for 8 h.The interactiveeffects were recorded on plant biomass, biosynthesis of photosynthetic pigments, proline, oil contents and flavonidsin Carum carvi plant. From our present results, itcouldbe concluded thatseed presoaked applications of ascorbic acid orα tocopherol could play a role to alleviate the harmful effect of lead stress on some metabolic and physiological processes of Carum carvi that reflected in, increasing fresh-dry matter of different organs, inducing a significant stimulatory effect of carotenoid contents, increasing oil,proline and flavonoid contents.https://ejbo.journals.ekb.eg/article_3613_140c619b6e3bc67775765c21fe22171c.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Purification and Characterization of Lignin Peroxidase Isozymes from Humicola grisea (Traaen) and Its Application in Bioremediation of Textile Dyes335343361410.21608/ejbo.2017.775.1045ENHany MoubasherBotany & Microbiology Department, Faculty of Science, Cairo University, Cairo, EgyptFatma AbdelazizMostafabotany and microbioogy department, faculty of science, cairo universitySalwa WahshFaculty of Science,Fayoum University,Fayoum, EgyptOmima HarounphD candidateJournal Article20170318PEROXIDASES have numerous important applications in industries and biodegradation of organopollutants , so there is a need to explore more and more sources of enzymes with different characteristics. In the present study, lignin peroxidases (LiPs) producing by fungus Humicola grisea were extracted and purified using gel filtration and ion exchange chromatography. Protein and heme protein were eluted in different peaks about 6 of these peaks had LiP activity and this is an indication that LiP in H. grisea was isoenzymes. Four of these isoenzymes were characterized regarding their molecular mass, optimum temperature and optimum pH for activity, substrate concentration and effect of some metal ions. The four purified isoenzymes have maximum activities at different temperatures, where optimum temperature for H2 and H4 isoenzyme were at 60 ºC, and the optimum temperatures for H1 and H3 were at 40 ºC and 30 ºC, respectively. The maximum activities of the four isoenzymes were within the acidic range. The different LiP isoenzymes showed different Km and Vmax values representing the varied affinity of the four isoenzymes using veratryl alcohol as a substrate. Km values for H1, H2, H3 and H4 were 5.26, 5.0, 1.3 and 1.4, respectively. While Vmax values for the same isoenzymes were 1.25, 0.8, 0.87 and 1.1, respectively.https://ejbo.journals.ekb.eg/article_3614_f27d0dc4c3b04d3f7bdd64fe12d1dfcf.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Biosynthesis of Silver Nanoparticles by Pathogenic and Nonpathogenic Strains of Fusarium oxysporum f. sp. lycopersici 345350361510.21608/ejbo.2017.789.1048ENFatma AbdelazizMostafabotany and microbioogy department, faculty of science, cairo universityJournal Article20170321N ANOTECHNOLOGY, a highly promising discipline in science and technology, is the emerging and novel trend that will redesign the future of several existing know how which will change every aspect of our lives and leads to the generation of uniqueness in all the streams of technology. Biological synthesis of metal nanoparticles using microbes is gaining momentum due to the ecofriendly nature of the organisms which reduce toxic chemicals. Plant pathogenic fungi produce extracellular degradative enzymes that may be important in pathogenicity. Biosynthesis of silver nanoparticles was investigated using pathogenic Fusarium oxysporum f. sp. lycopersici and nonpathogenic strains of the same fungal species. These silver nanoparticles were characterized by means of UV–vis spectroscopy, transmission electron microscopy (TEM). UV–visible spectrum of the aqueous medium containing silver ion showed a peak at 420 nm corresponding to the Plasmon absorbance of silver nanoparticles. Transmission electron microscopy (TEM) micrograph showed formation of well-dispersed spherical silver nanoparticles with mean sizes 11.20 nm and 15.38 nm for pathogenic and nonpathogenic strains, respectively.https://ejbo.journals.ekb.eg/article_3615_5003fb74c471489825f311a948824d9e.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Genetic Diversity and Inbreeding Level of Cotoneaster orbicularis Schltdl. in The Sinai Mountains Revealed by Microsatellite Markers and Flow Cytometry351361370410.21608/ejbo.2017.694.1037ENHassan MansourDepartment of Botany, Science faculty, Suez Canal UniversityElwira SliwinskaDepartment of Plant Genetics, Physiology and Biotechnology, UTP University of Science and Technology, Kaliskiego Ave. 7, 85-789 Bydgoszcz, PolandJournal Article20170224COTONEASTER orbicularis Schltdl, native to Egypt and the eastern part of the Arab peninsula, is an endangered species and the surviving individuals are dispersed in the mountains of South Sinai, Egypt. Several threats, including habitat aridification and the impact of human interference have caused a noticeable reduction in population size and number. Analyzing the genetic diversity and mating system of C. orbicularis is crucial for its conservation and management. In this study, six polymorphic microsatellite markers, formerly developed as species-specific markers, were used to assess the genetic variability and extent of inbreeding in the C. orbicularis populations. Flow cytometry of seeds was used to establish genome size. Low total heterozygosity (HT = 0.464) for all loci and populations, high differentiation between populations (FST = 0.634, RST = 0.889), and high levels of inbreeding (F = 0.906) were found. Most of the embryos possessed about 1.9 pg/2C DNA; however, embryos with 3.8 pg/2C DNA also occurred. The results provide markers that can be used in the management of conservation, including habitat recovery and ex-situ conservation, in order to prevent extinction.https://ejbo.journals.ekb.eg/article_3704_aa327a16a1a09b2f2e9dc62b441c760a.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Fenugreek Seed Extract Enhanced the Growth of Vicia faba and Zea mays Seedlings363377361710.21608/ejbo.2017.777.1047ENMahmoud MadanyDepartment of botany and microbiology, Faculty of science, Cairo universityRadwan RKhalilDepartment of Botany, Faculty of Science, Benha University, Benha, Egypt.https://orcid.org/00Journal Article20170319HIGHER plants produce many phytochemical constituents that not only play a pivotal role in plants producing them, but also affect their neighboring plant communities. This study was conducted to evaluate the effect of fenugreek seed extract on the growth of faba bean (Vicia faba) and maize (Zea mays) seedlings. The allelochemical components of fenugreek seed extract were analysed and confirmed the abundance of vanillic, syringic, 4-hydroxycinnamic, sinapic, caffeic, salicylic and p-hydroxybenzoic, ferulic acids as well as coumarin. Fenugreek seed extract up to 1.0 % (w/v) efficiently enhanced the growth as well as the chlorophyll content of faba bean and maize seedlings. Furthermore, elevated levels of the amylolytic and proteolytic activities under these concentrations of fenugreek treatments were associated with a marked accumulation of soluble sugars and proteins. Moreover, the potentiality of fenugreek treatments to accumulate phenolic and flavonoid was associated with stimulation in the activities of phenylalanine ammonia lyase, peroxidase and polyphenol oxidase as well as a marked enhancement in total antioxidant capacity which would improve the plant antioxidant status. Concerning the membrane integrity, fenugreek treatments caused a pronounced retardation in both MDA and H2O2 content. Our results elucidated the effect of fenugreek seed extract on the growth and some physiological behaviors of both faba bean and maize seedlings.https://ejbo.journals.ekb.eg/article_3617_1f715a34d7dc8431e0046595b26aaf63.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Morphological and Molecular Differentiation in Populations of Persian Oak ( Quercus Brantii Lindl.) in Southwestern Iran379393353710.21608/ejbo.2017.747.1042ENAkram EbrahimiDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranTaher Nejad SattariDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranMostafa AssadiResearch Institute of Forests and Rangelands, National Botanical Garden of Iran, Tehran, IranKambiz LarijaniDepartment of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.Iraj MehreganDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranJournal Article20170309PERSIAN oak (<em>Quercus brantii</em> Lindl.) is a common woody species in the Zagros forests of Iran. The current study evaluates the variation among the Q. brantii populations in the southwestern forests of Iran using amplified fragment length polymorphism (AFLP) and leaf morphology. A total of 135 trees were sampled from 27 populations representing nine regions in the provinces of Khuzestan (GOL, EMA, BAB, MOG, ALG), Chaharmahal and Bakhtiari (MON), Lorestan (KHA), Kohgiluyeh and Boyer-Ahmad (DIS) and Fars (BAA). Twelve morphological leaf traits were analyzed using PCA and Ward’s clustering methods. The results of ANOVA showed significant differences between populations, but the PCA graph and clustering analysis could not separate the populations on the basis of leaf characteristics. An analysis of molecular variance indicated that most genetic variation was contained within populations (95%); differences between populations accounted for only 1% and 4% was attributed to variation between regions. The five regions of Khuzestan have similar genetic structures. This was observed for the regions in Fars, Lorestan and Chaharmahal and Bakhtiari. Region DIS of Kohgiluyeh and Boyer-Ahmad had a separate genetic structure. The Dendrogram of Quercus brantii populations based on AFLP marker and Cluster analysis of populations using Ward’s method based on morphological data don’t confirm each other and in both, populations mix together. The current study revealed the some morphological and molecular differences in some populations of Persian oak in Iran. Population genetic information can provide critical insights into range expansion and evolutionary potential to adapt to environmental changes.https://ejbo.journals.ekb.eg/article_3537_97c7f7413bee1ada4a3b9cf48cf9e67b.pdfNational Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757220170801Studies on the Sclerotia of Some Species in The Genus Aspergillus 395404362810.21608/ejbo.2017.683.1036ENSoad Abu El-SouodBotany Department, Faculty of Science, Tanta University, Tanta, Egypt.Omyma AhmedAwadallaBotany Department, Faculty of Science, Tanta University, Tanta, Egypt.Susan Mohammed WagdyAssawahBotany Department, Faculty of Science, Tanta University, Tanta, Egypt.Yehia Abdel-GaleleMahmoudBotany Department, Faculty of Science, Tanta University, Tanta, Egypt.0000-0002-7009-0115Samah Abd El-KaderEl-DebaikyBotany department, Faculty of science, Tanta University, Tanta, EgyptJournal Article20170223THIS study is part of a large study concerning with the sclerotia of some species of genus<em> Aspergillus</em>. In the present study, the sclerotia of different aspergilli (<em>Aspergillus sclerotioniger, A. sclerotiorum, A. candidus, A. flavus, A. piperis, A. ochraceus, A. robustus, A. sepultus, A. petrakii, A. melleus, A. parasiticus </em>and<em> A. sclerotiicarbonarius</em>) were examined concerning their morphological and anatomical characters. The mature sclerotia in the cultures of the used aspergilli appeared with different shapes (globose, sub-globose and oval). Transverse sections of mature sclerotia revealed two regions; the outer region, considered as rind, while the inner region proposed as medulla. These aspergilli were tested also by polymerase chain reaction (PCR) technique for the presence of sclerotium regulator (SclR) gene. The results indicated that eight of them contain this gene; these were<em> A. petrakii, A. sepultus, A. robustus, A. sclerotiicarbonarius, A. melleus, A. sclerotioniger, A. parasiticus</em> and<em> A. sclerotiorum.</em> These species exhibited bands with different molecular weights when tested for presence of SclR gene. Each Aspergillus species showed one band with specific molecular weight, these bands ranged in size; the highest was 436 bp (<em>A. parasiticus</em>) while the lowest was 134 bp (<em>A. sclerotiicarbonarius</em>).https://ejbo.journals.ekb.eg/article_3628_204133c96a040bad641feb427348101b.pdf