National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Nutritive Values of Some Wetland Plants in the Deltaic Mediterranean Coast of Egypt110332410.21608/ejbo.2017.3324ENElsayed NafeaAquatic Environment Department, Faculty of Fish Resources, Suez
University, EgyptJournal Article20160826THIS STUDY aims to investigate the nutritive values of some wild<br />plants in the coastal Deltaic Mediterranean wetlands of Egypt for<br />sustainable uses and management; these are: Arthrocnemum<br />macrostachyum (Moric) Koch, Atriplex portulacoides L., Bassia indica<br />(Weight) A. J. Scott, Chenopodium album L., Halocnemum strobilaceum<br />(Pall.) M. Bieb (Chenopodiaceae) , Amaranthus hybridus L<br />(Amaranthaceae), Portulaca oleracea L.(Portulacaceae), Bolboschoenus<br />glaucus L.(Cyperaceae) , Sesbania sesban L. (Fabaceae ) and Pluchea<br />Dioscorides (L.) DC. (Asteraceae) as their over growth and domination<br />cause many serious environmental problems, So it is urgent to suggest<br />the possible economic uses of these plants as a tool in sustainable<br />management. The mean values of the physicochemical characteristics of<br />soil samples collected underneath the studied species were carried out to<br />be considered during cultivation of these plants. The determination of<br />lipid, crude protein, total phosphorus, total carbohydrates, crude fiber, ash<br />content and dry weight, as well as Na+, K+ and Ca+ cations in the dry<br />mater showed noteable variation among the studied species. The highest<br />mean carbohydrates content was recorded in P. oleracea and A. hybridus<br />with values of 211.3 and 291 mg/g dry wt. , respectively. The relatively<br />high contents of carbohydrates, lipids, and proteins in some selected plant<br />species qualify them as suitable candidates to be used as animal feed<br />stalk and natural organic fertilizers with safe manner. The high contents<br />of Na+, K+ and Ca+ ions in the studied plants make them also as a good<br />source of mineral salts in food formulas, and as a tool for desalinization in<br />the salt affected lands in the coastal Egyptian wetland habitats.<br /><br />National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Ameliorative Effect of Salicylic acid on Growth, Minerals and Nitrogenous Compounds of Vicia faba L. Plants under Salt Stress1129332510.21608/ejbo.2017.212.1003ENMarwa Mohame RagaeyMahmoudBotany Department, Faculty of Science, Assiut University, New Valley Branch, EgyptJournal Article20160830This study aimed to identify the salt tolerance of three broad bean genotypes (Vicia faba L.) and to elucidate the stimulatory role of salicylic acid on the studied bean genotypes under salt stress. Growth, photosynthetic pigments, minerals, membrane integrity and some nitrogenous compounds were evaluated. The three studied broad bean genotypes varied in their salt tolerance and in their response to salicylic acid application. Salinity reduced the shoot growth, the photosynthetic pigments, K+ contents and total proteins while it improved proline, total amino acids, soluble proteins and Na+ contents. Increasing the NaCl-salt level caused more cell membrane injury and electrolyte loss. The Na+/K+ ratio increased with increasing salt concentration in old leaves than in young leaves of bean genotypes. Foliar application of 0.5 mM salicylic acid to the bean genotypes under the different salinity levels improved these attributes significantly. Exogenous salicylic acid succeeded to ameliorate the oxidative stress in bean salt-stressed genotypes especially at the most sensitive genotype Assiut84/4. It can be concluded that broad bean plants could be possibly cultivated in moderate saline soils due to its capacity for osmotic adjustment. Moreover, salicylic acid could be used as a promising compound in broad bean cultivation under salt stress conditions.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Gene Over-Expression of Glycerol-3-phosphate Dehydrogenase in Some Marine Algae of Egypt.314897710.21608/ejbo.2017.977ENHoda AnwerMansourFaculty of Science, Ain Shams Univ.,Botany Dep., PhycologyManal EmmamFac. Sci., Ain Shams Univ., Bot. Dep.Journal Article20160831Studies focusing a survey or a comparison between freshwater and marine algae are relatively Algae exhibit a wide range of acclimation in response to induced salinity. However, studies focusing a survey or a comparison between freshwater and marine algae are relatively scarce. A comparative study between three fresh water algae (Spirogyra sp., Chara sp. and Cladophora sp.) and six marine algae among them brown: Sargassum dentifolium, Padina boryana, Dictyota dichotoma; red: Gelidium latifolium, Gracilaria dura and green: Enteromorpha intestinalis) has been performed to study how marine algae can cope with salinity of seawater. Algal fluorescence from the marine and fresh water groups was analyzed to determine whether differences in room-temperature fluorescence could be ascribed to differences between algal classes or specific for individuals. Results obtained showed that fluorescence signatures were relatively uniform for species within class but were different for different classes.<br /> Scanning of the SDS-gel showed the presence of certain characteristic protein banding pattern for each species. The amount of proline showed irregular differences according to species. Mannitol was detected only in the members of brown algae. Moreover, the mineral (sodium and potassium) and glycerol contents of the marine algae has noticeable greater values than those of fresh water members. The expression level of G3PDH mRNA was significantly upregulated in marine algal group as compared to that of the freshwater algae. GPDH had a significant impact on numerous metabolic processes in marine algae, including the biosynthesis of glycerol.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Dyes bio-sorption by two marine algae and their applications on industrial effluents from Borg El-Arab region, Egypt4973332610.21608/ejbo.2017.235.1005ENHoda AnwerMansourFaculty of Science, Ain Shams Univ.,Botany Dep., PhycologyYassmin Abou El-soud) Desert Research Center, Plant Ecology and Range Department, Cairo, EgyptJournal Article20160918ABSTRACT<br /> Batch biosorption experiments were carried out for the removal of four dyes; Drimarene yellow CL4R (Y), Drimarene blue K2RL (B), Congo Red (CR) and Malachite Green (MG) from their aqueous solutions using two raw algal biomasses namely Gelidium latifolium (Grev.) Bornet et Thuret (a red alga) and Ulva lactuca Linnaeus (a green alga). Additionally, the studied algal biomasses were applied under the optimum concluded conditions to treat real polluted industrial effluents from Borg El-Arab region, Egypt. Meanwhile these algal biomasses before and after the biosorption process were characterized using SEM and FTIR spectroscopy. Ulva lactuca has shown much better dyes biosorption efficiency particularly with solid/liquid ratio: 1/1000 g/mL, initial dye concentration: 400 mg/L, temperature: 40° C, contact time: 1 hour, pH: 1, 2, 8 and 7 for Y, B, CR and MG, respectively. This biosorption was nonspontaneous, endothermic and chemical in nature. Freundlich isotherm model fitted slightly better than the Langmuir model indicating multilayer coverage (heterogeneous sorption). The applied decolourization of coloured samples reached 97.46 %. <br /> We concluded that, algal biomasses of Ulva lactuca followed by Gelidium latifolium showed promising results in the field of water treatment and removal of water coloring. Beside that, the studied algal biomasses are considered as natural, low-cost and eco-friendly tool, so that they can help to a great extent in improving quality of water resources and to keep a clean environment via elimination of industrial pollution and its subsequent hazards on all biota especially human being.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Some Biofertilizers Relieved the Stressful Drawbacks of Calcareous Soil upon Black Seed (Nigella sativa L.) Through Inhibiting Stress Markers and Antioxidant Enzymes with Enhancing Plant Growth7592332710.21608/ejbo.2017.262.1006ENManal El-ZohriBotany and Microbiology Department, Faculty of Science, Assiut University, Assiut, EgyptNoha MedhatMedicinal and Aromatic Plants, Horticulture Research Institute, Agriculture Research Center, EL-Giza, EgyptFull-Elnada MSalehMedicinal and Aromatic Plants, Horticulture Research Institute, Agriculture Research Center, EL-Giza, EgyptSaad S.M.El-MaraghyDepartment of Botany and Microbiology, Faculty of Science, Assiut University, Assiut 71516, EgyptJournal Article20161005Calcareous soils have stressful properties to plant growth; they represent about 25-30% of the Egyptian total area. To improve soil characteristics, and hence plant growth, a number of biofertilizers (diatomaceous earth, Nostoc, sewage effluent and yeast) in addition to Hoagland medium were applied at successively increasing concentrations. Growth of Nigella sativa (black seed), a folk medicinal plant famous for its oils, was enhanced by all the applied treatments. Enhancement in plant biomass showed highest levels at a certain concentration of each treatment; sewage (20%) followed by diatomaceous earth (6g/5Kg), Nostoc (1mg/5 Kg), yeast (4*1012 cell/L) and Hoagland (1/4x). The applied treatments variably altered carbohydrates, proteins and amino acid proportions in plant biomass. Stress marker (malondialdehyde and proline) contents and antioxidant enzymes activity (superoxide oxidase, catalase and ascorbate peroxidase) have been all significantly reduced in shoots and roots of the treated plants, compared with those of the control plants grown on calcareous soil alone. Subsequently, it could be inferred that calcareous soil triggered oxidative stress and the applied biofertilizers extinguished it and overcame the stressful drawbacks of calcareous soil in terms of enhanced plant growth. Energy and carbon skeletons that are usually leaked into defense molecules (MDA, proline and antioxidant enzymes), have been saved for growth upon amendment of the stressful calcareous soil.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Air microbial contamination and factors affecting its occurrence in certain book libraries in Egypt.93118332810.21608/ejbo.2016.277.1007ENMohamed El-SayedOsmanBotany Dept., Faculty of Science, Helwan Univ., EgyptAbdel Hameed Abdel HameedAwadAir Pollution Dept., National Research Centre, Dokki, Giza, EgyptYasser HassanIbrahimAir Pollution Dept., National Research Centre, Dokki, Giza, EgyptYoussef FawzyAhmedAnimal Reproduction and A.I. Dept., National Research Centre, Dokki, Giza, EgyptAmany Abo-ElnasrBotany Dept., Faculty of Science, Helwan Univ., Egypt0000-0002-8954-0358Yuosra SaeedAir Pollution Dept., National Research Centre, Dokki, Giza, EgyptJournal Article20161013Bacterial and fungal contamination in the air and settled dust were studied in old and new book libraries located in the National Research Center (NRC), Dokki, Giza, Egypt. The investigated libraries differ in age, design, size, ventilation type, and number of occupants in relation to microclimatic parameters and particulate matter (PM) load. Airborne microorganisms were collected using an Andersen two stage impactor sampler. Indoor airborne bacteria and fungi ranged from 0-1060.4 CFU/m3 and 11.8-315.6 CFU/m3, respectively. Outdoor airborne bacteria and fungi ranged within 11.7-2514.7 CFU/m3 and 0-713.7 CFU/m3, respectively. Bacteria and fungi associated surface settled dust ranged from 0.4-10x106 CFU/gm and 0-73x104 CFU/gm, respectively. Fine microbial fraction (particles ≤ 8 μm in size) constituted 2-24.94% and 68.35-94.15% of the total airborne bacterial and fungal concentrations, respectively. Indoor/outdoor (I/O) ratios of airborne microorganisms were less than 1 at both libraries, indicating no indoor microbial sources. Gram positive cocci (14.3-47%) and bacilli (52.9-85.7%) were the dominant bacterial isolates in the air state, while bacilli represented 100% of the total isolates in the surface settled dust. Bacillus pseudomycoides and B. subtilis dominated indoors while B. subtilis and Staphylococcus outdoors. Aspergillus and Penicillium, were the common fungal species in both libraries under investigation. Many of the isolated fungal taxa had enzymatic activities (lipase, protease and cellulase), with A. flavus, Curvularia pallescens, Fusarium oxysporium, P. notatum and Trichoderma viride presented all enzymatic activities. Complex correlations and no-clear patterns were found between the airborne microorganisms and the environmental factors.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Fungi Associated with Maize and Sorghum Grains and their Potential for Amylase and Aflatoxins Production119137134610.21608/ejbo.2017.296.1008ENMohamed Abdel- SaterBotany and Microbiology Department Faculty of Science
Assiut University
Assiut,
EgyptSobhy Abdel- HafezBotany and Microbiology Department, Faculty of Science,
Assiut University,
Assiut,
EgyptNemmat HusseinAssiut University, Faculty of Science, Botany &amp; Microbiology DepartmentEshraq Al-ameryBotany and Microbiology Department, Faculty of Science,
Assiut University,
Assiut,
EgyptJournal Article20161019USING direct-plating technique, thirty-nine species belonging to<br /> 16 fungal genera were isolated from maize and sorghum grain<br /> samples (20 samples each) on Czap ek’s agar (Cz) and Czap ek’s<br /> supplemented with 40% sucrose agar (Cz40S) media at 28ºC. Widest<br /> spectrum of genera and species were recorded on sorghum (16 genera<br /> and 35 species) compared with maize grains (8 and 19). The highest<br /> total counts (139 CFUs/ 100 grains) and the number of genera (14)<br /> and species (27) were identified from sorghum grains on Cz medium.<br /> The most common fungi on the grains tested were Aspergillus flavus,<br /> A. niger, Eurotium amstelodami, E. rubrum, E. repens, Fusarium<br /> verticillioides and Rhizopus stolonifer. Among 129 isolates screened<br /> for their abilities to produce amylase enzyme, 102 isolates could<br /> produce this enzyme, of which A. terreus exhibited the highest<br /> production (EI=1.73). HPLC analysis revealed that out of 6 strains of<br /> A. flavus tested, strain No. AUMC 11311 showed the highest<br /> production of aflatoxin B1 and B2, while the highest value of aflatoxin<br /> G2 was produced by strain No. AUMC 11317. It could be concluded<br /> that fungi growing on grains and their abilities for producing enzymes<br /> and aflatoxins might cause deterioration and spoilage of these grains.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Removal of Some Pharmaceuticals and Endocrine Disrupting Compounds by the Marine Macroalgae Pterocladia capillacea and Ulva lactuca139155236310.21608/ejbo.2017.350.1009ENSoad MohamedMohy El.DinFaculty of Science - Alexandria UniversityNadia HegazyNoamanFaculty of Science -Alexandria UniversitySeham HossnyZakyFaculty of Science - Alexandria UniversityJournal Article20161105Phytoremediation of waters by aquatic organisms such as algae has been recently explored for the removal of organic pollutants possessing endocrine disrupting capacity. Two marine microalgae Pterocladia capillacea and Ulva lactuca were tested for tolerance and removal of chloramphenicol, clofibric acid, acetyl salicylic acid, nonylphenol and bisphenol in aqueous solutions as a single compound species at concentrations 5-60 mg/L under controlled laboratory conditions. The obtained results showed that chlorophyll “a” content of both algal biomasses reduced with increasing pharmaceuticals concentrations. Chlorophyll “a “content diminished nearly to half at 45mg/l for both algal species. However, the nonylphenol and bisphenol were showed a strong inhibition of chlorophyll “a” biosynthesis at higher concentration s (50-60 mg/L). The results also revealed that both the tested algae had high capacity for bioremoval of five pharmaceuticals which, recorded in order acetyl salicylic acid > bisphenol > clofibric acid > nonylphenol > chloramphenicol. Also Pterocladia capillacea had high capacity for bioremoval of five pharmaceuticals than Ulva lactuca. The highest percentage of pharmaceuticals occurred at 12 hours of contact. Both the tested algae suffered from oxidative stress as a result of drug exposure. Our results showed elevation in the antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APO), catalase (CAT) levels in the tested algae after exposure to different pharmaceuticals when compared with their activities in the control.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Foliar Application of Fresh Moringa Leaf Extract Overcomes Salt Stress in Fenugreek (Trigonella foenum-graecum) Plants157179263010.21608/ejbo.2017.317.1011ENArafat Abdel HamedAbdel LatefBotany Deptartment-Faculty of Science-South Valley University0000-0001-5244-4832Mona Abu AlhmadBotany Department, Faculty of Science, South Valley University, Qena 83523, EgyptSabah AhmadBotany Department, Faculty of Science, South Valley University, Qena 83523, EgyptJournal Article20161123Fresh moringa leaf extract (MLE) was applied and its impact was assessed on growth as well as some physiological and biochemical parameters of fenugreek (Trigonella foenum-graecum) grown under four levels (0, 50, 100 and 200 mM) of NaCl. Salinity decreased growth criteria, the contents of photosynthetic pigments, organic solutes (except proline), total phenols, K+, Ca2+, Mg2+, the ratio of K+/Na+ and Ca2+/Na+ and peroxidase (POD) activity. On the other hand, salinity stress boosted the contents of proline, Na+, malonaldehyde (MDA), the activity of catalase (CAT) and ascorbate peroxidase (APX). Interestingly, superoxide dismutase (SOD) activity remained unchanged under salinity levels. Foliar application of MLE ameliorated the negative impact of salinity to considerable extent by enhancing growth traits and all above parameters except Na+ and MDA. Under saline concentrations, foliar application with MLE led to the appearance of new 12 polypeptides. The 39, 21, 19, 17 and 16 kDa protein bands that were absent under the influence of salinity occurred under the combined effect of salinity and MLE. These results proposed that, foliar application of MLE could mitigate the harmful effect of salinity on fenugreek and the strategy may be employed to enhance the crop production in saline soils.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170426Size distribution of some endangered plant species, Al-Jabal Al-Akhdar, Libya.181197250010.21608/ejbo.2017.399.1012ENHosny Abd El-AzizMosallamBotany Department- Faculty of Science- Ain Shams UniversityAhmed HashimMohamedDepartment of Botany, Faculty of Science, Ain Shams University, Cairo, EgyptSalah SalemSergiwaDepartment of Agriculture, Faculty of Science, Omer Al-Mukhtar University, El-Beida, LibyaMabrooka Abd-AllahGibreelDepartment of Botany, Faculty of Science, Benghazi University, Benghazi, LibyaJournal Article20161123Al-Jabal Al-Akhdar Mountain is the richest vegetation and highest species diversity in Libya. The aim of the present study was to evaluate the population structure of twelve endangered species in Al-Jabal Al-Akhdar. These species are: Arbutus pavarii Pamp., Cupressus sempervirens L., Ceratonia siliqua L., Juniperus phoenicea L., Laurus nobilis L., Olea europaea L., Pinus halepensis Miller., Pistacia lentiscus L., Querecus coccifera L., Seriphidium herba-alba (Asso) Soják., Thymus capitatus (L.) Hoffmanns. & Link and Ziziphous lotus (L.) Lam. These species collected from downstream, midstream and upstream of Al-Marj-Al-Baida motorway, El-Beida, Jardas Jerrari, Shahat, Sidi Ahmad Al-Hemery, Susah and Wadi El-Kouf in Al-Jabal Al-Akhdar. The size index of each individual was calculated and then used to classify population into seven size classes. The height, mean diameter, height to diameter ratio, size index and volume per individual in each size class were determined. Generally, the height to diameter ratio was more than unity for Arbutus pavarii, Cupressus sempervirens, Ceratonia siliqua, Juniperus phoenicea, Laurus nobilis, Olea europaea, Pinus halepensis and Querecus coccifera, this means that the diameter of these species tend to expand vertically rather than horizontally, while the height to diameter ratio was less than unity for Pistacia lentiscus, Seriphidium herba-alba, Thymus capitatus and Ziziphus lotus. Five forms of size distributions along the different elevations were recognized: more or less inverse J-shaped distribution, positive skewed distribution, bell shaped distribution, more or less J-shaped distribution and more or less stationary size distribution biased to large size.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Effect of Gamma-radiation Doses of Phenotypic and Molecular Characteristics of two Egyptian Soybean Varieties199216233010.21608/ejbo.2017.431.1014ENReda GaafarBotany Dept., Tanta University0000-0003-0737-0605Adel ElshanshoryBotany Dept., Fac. of Science Tanta UniversityMarwa HamoudaBotany Dept., Fac. of Scinece, Tanta UniversityRana DiabBotany Dept., Fac. of Science, Tanta universityJournal Article20161202Two Egyptian soybeans varieties, Giza111 and Crawford were selected for the current study. Their seeds were exposed to three doses of gamma-radiation 150, 200, and 250 Gy in order to study their mutagenic effects in the treated seeds and two following generations at various levels. In the first generation (M1), two doses (150 and 200 Gy) were the most effective in enhancement of some agronomic traits like number of stem branches, number of pods per plant, number of seeds per pod and weight of 100 seeds and crop yield. <br /> Analysis of protein profiles using SDS-PAGE showed variation among the treatments in each variety, where a total of 38 protein bands were recorded. Three of which were unique bands, one unique band of size 37 kDa was found in the treated seeds before germination (M0) in Crawford at 250 Gy treatment. The other two unique bands (102 and 162 kDa) were found in M2 generation in Crawford at 150 Gy and Giza111 at 200 Gy, respectively.<br /> ISSR analysis using five primers gave a total 39 ISSR bands, 30 of which were polymorphic and 4 were unique bands. The primer HB14 amplified 6 DNA bands, 2 were unique bands (895 &1050 bp), both in M1 generation of Giza111 at 150 Gy, while primer 844 and 862 amplified only one unique band both found in M0 Giza111 control. They have a molecular size of 888 and 900 bp, respectively. Although, UBC-827 amplified the highest number of bands (8) no unique bands were found.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Evaluation of Elemental Sulphur Application with Rhizobia Inoculation on Peanut Yield and its Quality Grown in Sandy Soil at Egypt217240160310.21608/ejbo.2017.495.1017ENTharwat El-Sayed El-DesoukyRadwanEgyptian Society of BotanyJournal Article20161223 TWO field experiments were conducted during the summer of ….. two successive seasons of 2011 and 2012, to identify the response of peanut (<em>Arachis hypogaea</em> L., cv. Giza 5)to soil application of elemental sulphur (at rate of 15, 30 and 45 Kg s/fed before sowing) and Rhizobia(<em>Bradyrhizobium japonicum</em>) inoculation, individually or in combination, for determination of the yield criteria, pods yield, 100 seeds yield, seeds & straw yield and shilling percentage at harvest, as well as seed oil, oil yield, protein content and protein yield. The associated amelioration in plant macro and micronutrient contents and uptake, and soil biological activities (nodulation status, nitrogenase, dehydrogenase, CO<sub>2</sub> evolution and total bacterial counts) were assessed in this study. The obtained results indicated that<em> Rhizobium</em> inoculation or S addition alleviated the adverse effect of soil nutrient deficiency and caused significant increases in all the studied parameters of peanut and soil. <em>Rhizobium</em> inoculation individually caused insignificant increases in all the studied parameters over S addition solely at 45 kgs/fed. The joint addition of sulphur at all rates with <em>Rhizobium</em> inoculation gave high significant increases in all prior studied parameters and soil biological parameters at 50 and 80 days after peanut sowing followed by the individual treatments.<br /> <br /> <br /> <strong> </strong>National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Morphological VariabilityAmong Moringa oleifera (Lam.) Populations in Egypt.241257248310.21608/ejbo.2017.538.1020ENAhmed AbdelrazekMobarakBotany Department, Faculty of Science, Benha UniversityKamal ShaltoutTanta University, Egypt0000-0002-8588-2991Hussein IbrahiemAliFaculty of Agriculture, Alexandria UniversityDina BarakaBotany Department, Faculty of Science, Benha UniversitySadia AlyBotany Department, Faculty of Science, Benha UniversityJournal Article20170109This paper aims atstudying the morphological variation among Moringa oleifera populations in 8 sites in Egypt. The population of Qanatir Horticulture Research Institute had the maximum size (853.8 m3tree-1) and diameters of canopy (9.9 m tree-1) and stem at ground and breast height levels (76.1 and 63.4 cm tree-1), while those of Sheikh Zuweid had the minimum size (5.6 m3 tree-1) and height (2.0 m tree-1). Many branching modes of the main trunk were observed, where sympodial branching was the dominant in most sites. M. oleifera leaves in Botanic Garden of Faculty of Education - Ain Shams University were the longest (59.6 cm leaf-1) and those in Sheikh Zuweid were the shortest (24.5 cm leaf-1). On the other hand, the pods of Qanatir Horticulture Research Institute were the longest (50.4 cm pod-1). In addition, the pods of Botanic Garden of Faculty of Education - Ain Shams University contained the maximum number of seeds (21.8 seed pod-1), while the heaviest seeds were those of Sheikh Zuweid (270.0 mg seed-1). Variations in the morphology of M. oleifera organs (trees, leaves, pods and seeds) may be not only related to environmental conditions, particularly the soil properties, but also genetic differences within species. A further study on the genetic variation among the populations of this plant should be carried out.National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)Egyptian Journal of Botany0375-923757120170501Rise Potassium Content of the Medium Improved Survival, Multiplication, Growth and Scavenging System of in Vitro Grown Potato Under Salt Stress.259275165610.21608/ejbo.2017.642.1030ENAhmed MohamedHassaneinSohag University, Faculty of ScienceJehan SalemFaculty of Science, Sohag UniversityJournal Article20170205IN VITRO grown potato cultivars shoots were used to understand<br />….. how polyvinyl pyrrolidone (PVP) and three levels of potassium<br />interact with two levels of salinity to determine Na+ and K+ contents,<br />and antioxidant enzyme activities leading to control survival,<br />multiplication and growth of cultured plant shoots. Explant survival<br />and the number of regenerants/explant decreased with the increase of NaCl<br />concentration but increased K+ content of the culture medium from 20<br />mM to 30 mM improved explants survival frequency and the growth<br />parameters estimated under relatively high salt stress (80 mM NaCl) in<br />both cultivars. Also, number of regenerants was influenced by K+ level<br />where it was higher in medium containing 40 mM NaCl and 30 mM<br />K+ than other cultured on the same NaCl concentration with 20 mM<br />K+. The positive effect of 30 mM K+ on the previous parameters was<br />associated with increase shoot K+ content leading to decrease Na+/K+<br />ratio and increase of some antioxidant enzymes (SOD, POX and CAT<br />in Agria or SOD, POX and APX in Hermes) activity, especially under<br />relatively high salt stress. PVP application increased K+ content and<br />activities of some antioxidant enzymes (CAT and APX) but decreased<br />Na+/K+ ratio of shoots subjected to relatively high salt stress in Agria<br />but under both salt stresses in Hermes. Consequently, sufficient<br />potassium supply was necessary to conserve low Na+/K+ ratio and<br />efficient scavenging system by antioxidant agent (PVP) application or<br />increase the endogenous antioxidant enzymes activities leading to<br />minimize the negative effect of salt stress on in vitro grown potato.<br /><br />