@article { author = {}, title = {RAPD Based Genetic Diversity Analysis Within The Genus Solanum }, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {175-185}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.211}, abstract = {SOLANUM L., the largest genus of the Solanaceae family, varies …… morphologically and is ecogeographically distributed. In Saudi Arabia, previous studies for characterization and genetic variability of Solanum had focused mainly on chromosome morphology, genome description, and medicinal values, which are insufficient for genetic affinities. This investigation assessed molecular diversity of some species of Solanum from Taif highlands based on random amplified polymorphic DNA markers and proteins by SDS-PAGE. From 25 random primers investigated, 11 primers gave reproducible amplification banding patterns of 208 polymorphic bands scoring 100% polymorphism across the genotypes. Primer OPA-09 generated maximum polymorphic pattern, whereas primers OPP-10, OPN-13, and OPN-05 produced minimum polymorphism. The size of the amplified products varied from 265 to 2003bp. SDS denatured protein gels resolved 51bands most of them were not shared and some were unique and can be correlated with the DNA polymorphism. Based on these markers, genetic similarity coefficient was calculated and a dendrogram was constructed.}, keywords = {Solanum ,RAPD,Electrophoresis,genetic relationships}, url = {https://ejbo.journals.ekb.eg/article_211.html}, eprint = {https://ejbo.journals.ekb.eg/article_211_5a54454c0b5361cf0ab04be3f0079b3d.pdf} } @article { author = {}, title = {Bioremediation of Crystal Violet and Malachite Green Dyes by Some Algal Species}, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {187-196}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.212}, abstract = {BIOLOGICAL treatment of crystal violet and malachite green using blue-green algae (Nostoc sp., Microcystis aeruginosa and Oscillatoria geminata) and green algae (Chlorella vulgaris and Scenedesmus sp.) in order to assess the decolorization ability of these algae after incubation for 2 and 24 hr in three dyes concentrations at 10, 50 and 100 ppm. Chlorella has achieved the highest percentage of crystal violet decolorization after 2 hr at 10 ppm, but after 24 hr Scenedesmus gave the highest percentage of decolorization at 10 ppm. The highest proportion of malachite green decolorization after two hours using Chlorella at 50 ppm, but after 24 hr the higher decolorization percentage of malachite green obtained by M. aeruginosa at 100 ppm followed by Scenedesmus sp. at the same concentration. The green algae showed a high capacity for crystal violet decolorization than blue-green algae. Measuring the activity of laccase, manganese peroxidase and tyrosinase enzymes indicated that Nostoc sp. gave the highest laccase activity in all dyes concentrations, while the other two enzymes haven't any activity. Chlorophyll-a and phaeophytine-a values obtained showed significant differences between most treatments; the most negatively affected species was O. geminata which showed decreasing in chl-a content in the two dyes concentrations. In conclusion; the decolorization process of dyes by algae had been done by different mechanisms; one of them was enzymatic degradation as Nostoc sp. C. vulgarisand Scenedesmus sp. have a high ability to decolorize the two dyes so they might be used in wastewater treatment of fish farms contains these carcinogenic dyes as antifungal agents.}, keywords = {bioremediation,Triphenylmethane dyes,Malachite green,Crystal Violet,Cyanophyta,chlorophyta}, url = {https://ejbo.journals.ekb.eg/article_212.html}, eprint = {https://ejbo.journals.ekb.eg/article_212_8b5bcbf58236fa75b57a4b9eda8c7b9e.pdf} } @article { author = {}, title = {Effect of Cell - Free Cultures Filtrates of Different Bacterial Isolates on Seed Germination and Seedling Growth of Parkinsomia aculeata L.}, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {197-205}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.213}, abstract = {BACTERIAL species produce a variety of beneficial metabolites for plant growth and survival, as well as defend their hosts from attack of certain pathogens. The present study was carried out to investigate the impact of different bacterial isolates on the seed germination and seedling growth of Parkinsomia aculeata L. as a species of arid zones. Both soil samples and tested seeds were collected from El-Madina El-Monawara city, Saudi Arabia in May, 2013. Results evinced the promotion of seed germination by all bacterial isolates used. Isolates 6 and 7 (Micrococcous sp. and Sporosarcia sp.) , respectively isolated from soils of extremely low salinity recorded the highest values of germination percentage (44 and 48). On the same time, the greater seed vigor index (83.04), and energy of germination (1.96) were given by isolate 7 at the 7th day of the experiment. On the other hand, the highest R/S ratio (0.55), on the basis of length, and the highest dry weight of root and shoot were attained by Bacillus subtilis isolate.}, keywords = {Bacteria,Germination index,Filtrate,seedling vigor}, url = {https://ejbo.journals.ekb.eg/article_213.html}, eprint = {https://ejbo.journals.ekb.eg/article_213_15ad8b5055f046c924170a4f1fe93615.pdf} } @article { author = {}, title = {Pollen Morphology and Protein Pattern of Nitraria retusa and Some Selected Taxa of Zygophyllaceae in Egypt}, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {207-230}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.214}, abstract = {POLLEN morphology and pattern of Nitraria retusa (Forssk.) Asch. were studied and compared against eight selected taxa of Zygophyllaceae viz. Fagonia arabica, F. cretica, Peganum harmala, Tribulus terrestis, Zygophyllum album, Z. coccenium, Z. decumbens and Z. simplexusing Scanning Electron Microscope (SEM) and sodium dodecyle sulphate-polyacrylamide gel electrophoresis technique (SDS-PAGE). A wide range of measurements were obtained from digitized SEM images of whole pollen grains and exine pattern. Twenty nine protein bands were obtained with 100% polymorphism among the species examined.F. arabica and T. terrestis were characterized by one positive unique band with a molecular weight 56.8 and 24 kDa respectively.P. harmula was characterized by two positive unique bands with a molecular weight 62 and 42 kDa. N. retusa was characterized by three positive unique bands with amolecular weigh 64.6, 60.7 and 36 kDa. A dendrogram was constructed based on the similarity data matrix by unweighted pair group method using arithmetic averages cluster analysis. SEM and protein pattern analysis recommended the separation of Nitraria retusa (Forssk.) Asch. besides Fagonia sp. into a distinct family.}, keywords = {}, url = {https://ejbo.journals.ekb.eg/article_214.html}, eprint = {https://ejbo.journals.ekb.eg/article_214_396ec3cd9da503e81a72a76e9f0d212d.pdf} } @article { author = {}, title = {Biosorption of Iron from Ground Water by Biomasses of Yeast (Saccharomyces cerevisiae ) }, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {231-245}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.215}, abstract = {ELEVEN S. cerevisiae strains alive and dead forms were screened for biosorption and bioaccumulation of iron from prepared aqueous solution. S. cerevisiae strains F-707 in alive form was found to be excellent Fe+2 biosorbent that biosorbed 21.9 mg Fe+2/ g yeast biomass. Optimization of cultural conditions revealed that optimum concentration of Fe+2 was 5.6 mg Fe+2 /l after 20 min at pH 7, agitation rate ,150 rpm and yeast biomass concentration 0.1g / l at 30ºC for maximum Fe+2 biosorption by S.cerevisiae F-707 in alive form}, keywords = {Biosorption,iron,ground water,Saccharomyces cerevisiae. }, url = {https://ejbo.journals.ekb.eg/article_215.html}, eprint = {https://ejbo.journals.ekb.eg/article_215_f32855a6cf5f007f1c36237370fe57ea.pdf} } @article { author = {}, title = {Phenetic Analysis of Certain Taxa of Euphorbiaceae Grown in Egypt}, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {247-267}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.216}, abstract = {Euphorbiaceae is one of the major flowering plant families. Macro- and micromorphological as well as vein architectural characters of 34 taxa of Euphorbiaceae (14 genera, 29 species and eight varieties) were investigated. The studied taxa were collected from natural habitats and from different botanical gardens in Egypt. The macro-, micromorphological characters and vein architectural aspects were considered diagnostic characters which facilitate the separation between the taxa under investigation. The sum of 346 attributes were numerically analyzed using NTsys-pc program (version 2.02). The resulted phenogram is discussed showing two outgroups and five major clusters. The present study recommends the separation of Andrachne aspera and Putranjiva roxburghii in two separate families; Phyllanthaceae and Putranjivaceae respectively}, keywords = {Euphorbiaceae,Petiole,Vasculature,stomata,Vein architecture}, url = {https://ejbo.journals.ekb.eg/article_216.html}, eprint = {https://ejbo.journals.ekb.eg/article_216_b2cbe5d335ba335c9560d1978d5ecda5.pdf} } @article { author = {}, title = {Integration Effect Between Biofertilizers and Natural Fertilizers on Growth, Npk Uptake and Yield Parameters of Wheat Plant Grown in Alkaline Soil}, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {269-279}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.217}, abstract = {TE AIM of this study was to assess the effectiveness of mixed Bacillus strains applications. These strains are B. megaterium var. phosphaticum which efficient in dissolving phosphate, B. circulansas potassium release bacteria and B. polymyxa as nitrogen fixer. The effect of the applied mixed bacillus strains, natural fertilizers and their interactions on the growth, nutrients uptake of nitrogen, phosphorus and potassium, and yield parameters of wheat plants grown in a potted soil under natural conditions were studies. The results revealed that, the application of biofertilizers (Bacillus strains) with natural fertilizers (rock phosphate and feldspar) in alkaline soil caused a significant increase in growth and macronutrients (N, P and K) at vegetative stage which directly enhanced the yield parameters of wheat plants. Moreover, these dual fertilizers caused significant increase in ions (N, P and K) and crude protein contents of grains. It can be concluded that the combination between biofertilizers and natural fertilizers are one of the best modern tools and can replace the chemical fertilizers.}, keywords = { Bacillus megaterium,B. circulans,B. polymyxa ,Rock Phosphate,feldspar}, url = {https://ejbo.journals.ekb.eg/article_217.html}, eprint = {https://ejbo.journals.ekb.eg/article_217_998aead8f933f3172951bd7e18e2a5ec.pdf} } @article { author = {}, title = {Detection of Genotoxic Effect of Wastewater on Vicia faba L. by Using Biochemical Assay and RAPD Markers}, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {281-296}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.218}, abstract = {MULTIPLE biomarker systems have been frequently used to measure the genotoxic effects of environmental pollutants on living organisms. In this study, faba bean seedlings were used (Vicia faba) as a bioindicator for water pollution. Five different sources of water: distilled water as a negative control, tap water, and polluted water from three different sources were used in irrigation of bean plants. All treatments showed great alterations in seed proteins, isozyme systems (α- esterase, β- esterase, peroxidase, and acid phosphatase) and DNA polymorphism detected by RAPD analysis. The detected variations between treatments included alterations in number (appearance and disappearance), staining intensity, thickness and relative mobility of bands. These results suggest that the irrigation by wastewater can cause genotoxic effects on plant and V. faba can be used as a bioindicator for evaluation of environmental pollution.}, keywords = {genotoxicity,Isozyme,pollution,RAPD,SDS-PAGE,Vicia faba. }, url = {https://ejbo.journals.ekb.eg/article_218.html}, eprint = {https://ejbo.journals.ekb.eg/article_218_a5ce4714ba7fd7e1092aff57110bebe5.pdf} } @article { author = {}, title = {Role of Vesicular Arbuscular Mycorrhizal Inoculation of Zea mays on Heavy Metals Tolerance.}, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {297-306}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.219}, abstract = {THE STUDY evaluated the physiological responses of maize plant inoculated with vesicular arbuscular mycorrhiza (VAM) isolated from polluted site and growing in heavy metal (Cd or Pb) polluted soil. Chlorophyll, soluble carbohydrate, soluble protein, nutrient elements (P and N) content and phosphatase activity were analyzed. The phosphatase activity in inoculated plants was significantly increased in comparison to the non-inoculated ones. In addition, the contents of N, P, chlorophyll, total soluble carbohydrates and protein were significantly increased in plants inoculated with mycorrhiza grown in heavy metal ( Cd or Pb) contaminated soil in comparing with the non-inoculated ones. Therefore, it can be concluded that the VAM inoculation is capable to alleviate the damage caused by heavy metal (Cd or Pb) on maize plants that maintain the growth and this could be related to nutrient status offered by VAM. Isolation of heavy metal tolerant VAM fungi can be a potential biotechnical tool for inoculation of plant to enhance its heavy metal tolerance.}, keywords = {Heavy metals,VAM,Zea mays,Phosphatase}, url = {https://ejbo.journals.ekb.eg/article_219.html}, eprint = {https://ejbo.journals.ekb.eg/article_219_1aef6c4705d2acee7296ea17a2bf1c2c.pdf} } @article { author = {}, title = {Determination of Genetic Diversity of Some Species of Brassicaceae Using SDS-PAGE of Seed Protein and ISSR Markers}, journal = {Egyptian Journal of Botany}, volume = {55}, number = {2}, pages = {307-318}, year = {2015}, publisher = {National Information and Documentation Center (NIDOC), Academy of Scientific Research and Technology (ASRT)}, issn = {0375-9237}, eissn = {2357-0350}, doi = {10.21608/ejbo.2015.220}, abstract = {EVALUATION of genetic diversity among 10 species of Brassicaceae have been performed based on variations in seed protein electrophoretic patterns as revealed by SDS-PAGE and ISSR analyses, separately and in combination. The maximum genetic similarity was observed between Brassica nigra and B. rapa, while the lowest genetic similarity was observed between B. nigra and Sisymbrium irrio. Cluster analysis generated a dendrogram that separated the studied taxa in two main clusters; the five species of tribe Brassiceae were grouped close to each other in one cluster with Sisymbrium irrio. The remaining studied species; Capsella bursapastoris, Erysimum cheiranthoides, Mathiola longipetale and Thlaspi arvense, were grouped in a second cluster.}, keywords = {Brassicaceae,SDS-PAGE,ISSR,Genetic diversity}, url = {https://ejbo.journals.ekb.eg/article_220.html}, eprint = {https://ejbo.journals.ekb.eg/article_220_db2b59f6794b601cb085211dd449f2e8.pdf} }