Biological and Molecular Studies on an Egyptian Potyvirus Isolate from Hyocyamus muticus L.

Elbaz, Reham1* M.; Abd El-Salam, E.T.; Osman, M.E.; Abd El-Monem, Asmaa F.

doi:10.21608/ejbo.2017.1147

Biological and Molecular Studies on an Egyptian Potyvirus Isolate from Hyocyamus muticus L.

Authors

¹ Botany and Microbiology Department, Faculty of Science, Helwan University.

² Botany and Microbiology Department, Faculty of Science, Cairo University,

³ Botany and Microbiology Department, Faculty of Science, Helwan University

10.21608/ejbo.2017.1147

Abstract

ANEW isolate of potyvirus was isolated from naturally-infected
……. henbane plants (Hyocyamus muticus L.) collected from the
farm of Faculty of Pharmacy, Cairo University in January 2013. The
naturally infected henbane plants exhibited severe mosaic, rugosity,
blistering and malformation. Thermal inactivation point, dilution end
point and longevity in vitro of virus isolate were found to be 65 ºC,
10- 4 and 4 days, respectively. Electron micrograph of partial purified
virus negat ively s t ained wit h 2% p hosp hot unges t ic acid
revealed the presence of filamentous viruses with size 1440 X14.3nm.
Total RNA was extracted from infected henbane plant. Comparative
nucleotide sequence analysis for virus showed a high degree of
similarity (62 %) with four potyviruses accessions (EU482153: Potato
virus Y isolate Foggia, KF850513: Potato virus Y isolate M3,
AM184113: Henbane mosaic virus partial gene for polyprotein-
PHYS/H isolate, AY166867: Potato virus Y strain N isolate). The
virus isolate was published in GenBank with accession number
KM497011.

Keywords