Histo- and Cyto-pathological Alterations Induced by the New Egyptian Isolate Potyvirus on Hyocyamus muticus L. Leaves

Elbaz, Reham M.; Osman, M. E.; El-Monem, Asmaa F. Abd; Abd El-Salam, E.T.

doi:10.21608/ejbo.2017.1149

Histo- and Cyto-pathological Alterations Induced by the New Egyptian Isolate Potyvirus on Hyocyamus muticus L. Leaves

Authors

¹ Botany and Microbiology Department, Faculty of Science, Helwan University

² Botany and Microbiology Department, Faculty of Science, Cairo University

10.21608/ejbo.2017.1149

Abstract

HISTOPATHOLOGICAL and cytopahological studies on infected
Hyocyamus muticus L. plants with a new potyvirus-Egyptian
isolate with accession number KM497011 was investigated. The
infected Hyocyamus muticus L. showing virus-like symptoms on its
leaves in the form of a net mosaic, blistering, rugosity and
malformation. Histopathology of an Egyptian potyvirus isolateinfected
Hyocyamus muticus L. leaves using light microscopy showed
abnormal structures of the lamina, epidermal cells, mesophyll tissue
and vascular bundles compared with healthy one. Cytopathology of an
Egyptian potyvirus isolate-infected Hyocyamus muticus L. leaves using
electron microscopy showed pinwheel and scrolls inclusion bodies and
abnormal cell wall thickness, nucleus, chloroplast and no clear internal
structure of mitochondria compared with healthy one. Our conclusion,
the new Egyptian potyvirus isolate caused changes in the cells and
tissue structure of henbane leaves as the same of other known
potyvirus strains.

Keywords