Antimicrobial and In vitro

Lashin, Islam I.; Aref, Mohamed S.; Abd El-Kader, Esam M.; Heussin, Essam A.; Ewais, Emad El- Din A.

doi:10.21608/ejbo.2021.81121.1706

|
|
|
How to cite
RIS EndNote BibTeX APA MLA Harvard Vancouver
|
Share

	Antimicrobial and In vitroDillenia indica L.
Article 17, Volume 62, Issue 1, January 2022, Page 213-226 PDF (3.41 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2021.81121.1706
(5)
View on SCiNiTO
Authors
Islam I. Lashin ¹; Mohamed S. Aref¹; Esam M. Abd El-Kader²; Essam A. Heussin¹; Emad El- Din A. Ewais¹
¹Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Nasr City 11651, Cairo, Egypt
²Timber Trees Research Department, Horticulture Research Institute, Agriculture Research Center, 12619, Giza, Egypt
Abstract
IN THIS STUDY, we established of an application protocol for the micropropagation of Dillenia indica with an estimate of betulinic acid from callus, leaf, and regeneration plant. Moreover, the antimicrobial and cytotoxic efficacy of the crude extract and pure compound was compared. The highest number of shoots (3.667 shoots), leaf number per jar (27 leaves) and shoot length (3.667cm) were recorded from the MS medium containing 2.0mg L-1 of benzyl amino purine (BAP). Moreover, different physical elicitors (ultraviolet irradiation, microwave radiation, and light quality) were used on the production and increased the concentration of betulinic acid in callus of D. indica. The data revealed that the highest betulinic acid content (341.775mg/ 100g fresh weight) was recorded in callus microwaved with 200 watts for 10s. Which was three times more than the betulinic acid content recorded in the mother plant. In addition, different crude extracts of D. indica were used to determine their biological activity against six microorganisms. The data showed that the highest diameter of inhibition zones (17.0 mm) was recorded in callus culture extractions incubated under blue light with Candida albicans. The cytotoxic efficacy of betulinic acid and extract of callus, mother plant leaf or in vitro regeneration plants of D. indica were investigated against the Vero cell line by MTT. The results revealed that The IC50 values were 115.7 ± 9.17, 114.98 ± 5.2, 59.45 ± 3.06, and 72.76 ± 6.93μgmL-1 of betulinic acid, callus extract, leaf, and regeneration plant, respectively.
Keywords
Antimicrobial activity; Betulinic acid; Callus culture; Dillenia indica; Vero cell


Statistics Article View: 592 PDF Download: 961

Antimicrobial and In vitroDillenia indica L.