Mohammad, H., Tawfeek, F., Eltanahy, E., Mansour, T., Khalil, Z. (2023). Enhancement of Growth, Lipid, and Carbohydrate Production of the Egyptian isolate Dunaliella salina SA20 Using Mozzarella Cheese Whey as a Growth Supplement. Egyptian Journal of Botany, 63(1), 101-111. doi: 10.21608/ejbo.2022.145428.2020
Hend Y. Mohammad; Fatma El-Zahraa Tawfeek; Eladl Eltanahy; Tamer A. Mansour; Zeinab Khalil. "Enhancement of Growth, Lipid, and Carbohydrate Production of the Egyptian isolate Dunaliella salina SA20 Using Mozzarella Cheese Whey as a Growth Supplement". Egyptian Journal of Botany, 63, 1, 2023, 101-111. doi: 10.21608/ejbo.2022.145428.2020
Mohammad, H., Tawfeek, F., Eltanahy, E., Mansour, T., Khalil, Z. (2023). 'Enhancement of Growth, Lipid, and Carbohydrate Production of the Egyptian isolate Dunaliella salina SA20 Using Mozzarella Cheese Whey as a Growth Supplement', Egyptian Journal of Botany, 63(1), pp. 101-111. doi: 10.21608/ejbo.2022.145428.2020
Mohammad, H., Tawfeek, F., Eltanahy, E., Mansour, T., Khalil, Z. Enhancement of Growth, Lipid, and Carbohydrate Production of the Egyptian isolate Dunaliella salina SA20 Using Mozzarella Cheese Whey as a Growth Supplement. Egyptian Journal of Botany, 2023; 63(1): 101-111. doi: 10.21608/ejbo.2022.145428.2020
Enhancement of Growth, Lipid, and Carbohydrate Production of the Egyptian isolate Dunaliella salina SA20 Using Mozzarella Cheese Whey as a Growth Supplement
1Department of Botany and Microbiology, Faculty of Science, Cairo University, Giza, Egypt
2Department of Botany, Faculty of Science, Mansoura University, Mansoura, Egypt
3Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Mansoura, Egypt
Abstract
THE growth of Dunaliella salina SA20 (Dunal) Teodoresco Egyptian isolate (from El-Bardawil Lake, Sinai, Egypt) in wastewater is a more cost-effective cultivation method. Wastewater, such as mozzarella cheese whey (MCW), is produced in massive quantities as a dairy industry byproduct, and growing D. salina in such waste could be used safely in biotechnological applications. Applying different organic MCW ratios to inorganic Loeblich nutrient medium (20, 50, 75% v/v MCW/Loeblich) as growth supplement allowed mixotrophic nutrition, while growth in completely inorganic medium (100% Loeblich medium) allowed for autotrophic nutrition. The optimum growth performance of D. salina cells was observed in 20% MCW and was indicated by measured values of chlorophyll a (10.51mg/L), dry weight (405mg/L), cell count (6.92 x 106cell/ml), and optical density (1.12). Using 20% MCW, different pH values were applied to explore the pH effect on nutrient availability, and pH 7.5 was found to be optimal for growth. Additionally, a growth curve experiment comparing the mix- and autotrophic conditions presented higher values of the relative mean and maximum growth rates in mixotrophic conditions (0.19, 0.21 day−1, respectively) than in the autotrophic conditions (0.16, 0.18 day−1, respectively).
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