Impact of Thermotolerance Response on the Physiological and Genomic Variation of Fusarium oxysporum

Document Type : Regular issue (Original Article)

Authors

1 Agricultural Genetic Engineering Research Institute, Agricultural Research Centre, Giza 12619, Egypt

2 Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, Egypt

3 National Biotechnology Network of Expertise, ASRT

Abstract

Fusarium oxysporum strain F2 was isolated from the soil. It was morphologically and molecularly identified. The isolate showed promising thermotolerance characteristics. This was shown through the production of permanent proteins after 120 minutes of pre-thermal exposure at 40 ͦ C, as detected by SDS-PAGE. On the other hand, the RAPD marker revealed higher genetic variations produced by Fusarium oxysporum than the ISSR marker. Thirteen ISSR primers yielded 137 total bands, of which 81 were polymorphic bands with a percentage of 59.12%. While 18 RAPD primers yielded 223 total bands, 141 of which were polymorphic bands with a percentage of 63.23%. Notably, elevated temperatures triggered the isolate to enhance the production of hydrolytic enzymes. Fusarium oxysporum strain F2 produced more cellulolytic and pectinolytic enzymes until 120 min pre-thermal exposure at 40 ͦ C, displaying 3.262 and 1.797 (IU/ml), with corresponding percentages of activation 82.031 % and 73.288 %, respectively. This promising yield of cellulolytic activity could be employed in biofuel production.

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