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El-Taweel, N., Sadak, M., Khalifa, N. (2023). Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in Pisum sativum L.. Egyptian Journal of Botany, 63(3), 969-980. doi: 10.21608/ejbo.2023.202685.2295
Nada A. El-Taweel; Mervat Sh. Sadak; Noha S. Khalifa. "Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in Pisum sativum L.". Egyptian Journal of Botany, 63, 3, 2023, 969-980. doi: 10.21608/ejbo.2023.202685.2295
El-Taweel, N., Sadak, M., Khalifa, N. (2023). 'Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in Pisum sativum L.', Egyptian Journal of Botany, 63(3), pp. 969-980. doi: 10.21608/ejbo.2023.202685.2295
El-Taweel, N., Sadak, M., Khalifa, N. Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in Pisum sativum L.. Egyptian Journal of Botany, 2023; 63(3): 969-980. doi: 10.21608/ejbo.2023.202685.2295

Effect of Abscisic acid on Legume Growth Rate, Seed Development and Protein Profile in Pisum sativum L.

Article 19, Volume 63, Issue 3, September 2023, Page 969-980  XML PDF (2.45 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2023.202685.2295
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Authors
Nada A. El-Taweelorcid 1; Mervat Sh. Sadakorcid 2; Noha S. Khalifa email orcid 1
1Molecular Genetic Unit, Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt
2Botany Department, Agricultural and Biological Institute, National Research Center, Dokki, P.O. 12622, Giza, Egypt
Abstract
In this work, cultivated pea (Pisum sativum L.cv. Master B) is used as a legume model crop plant to investigate the effect of abscisic acid (ABA) treatment on seed filling during embryogenesis. This was evaluated by using two exogenously applied concentrations (10& 20 µM) during three stages of seed developments (early, intermediate and late). Phenotypic characterization of the treated plants included measurement of seed / legume length and diameter at full maturation stages after treatment. The expression of major proteins of the treated plants was detected using SDS-PAGE electrophoresis technique. Our data showed a positive effect of ABA when applied at the late stage of seed development. ABA significantly increased legume size and elevated the expression of some major proteins such as convicilin (70-75 kDa), vicilin (50, 34 kDa) and legumin (40 kDa). The impact of these results is further discussed. 
Keywords
ABA; Embryogenesis; Pisum sativum; SDS- PAGE; Seed filling
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