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Egyptian Journal of Botany
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(2016). Isozyme Variations in some Wheat Genotypes at Siwa Oasis and Ashmon Habitats. Egyptian Journal of Botany, 56(1), 1-14. doi: 10.21608/ejbo.2016.341
. "Isozyme Variations in some Wheat Genotypes at Siwa Oasis and Ashmon Habitats". Egyptian Journal of Botany, 56, 1, 2016, 1-14. doi: 10.21608/ejbo.2016.341
(2016). 'Isozyme Variations in some Wheat Genotypes at Siwa Oasis and Ashmon Habitats', Egyptian Journal of Botany, 56(1), pp. 1-14. doi: 10.21608/ejbo.2016.341
Isozyme Variations in some Wheat Genotypes at Siwa Oasis and Ashmon Habitats. Egyptian Journal of Botany, 2016; 56(1): 1-14. doi: 10.21608/ejbo.2016.341

Isozyme Variations in some Wheat Genotypes at Siwa Oasis and Ashmon Habitats

Article 1, Volume 56, Issue 1, September 2016, Page 1-14  XML PDF (787.22 K)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2016.341
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Abstract
      HE VARIABILITY of grain storage-proteins of twelve wheat …..genotypes under stress (Siwa Oasis) and normal (Ashmon) habitats were analyzed using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). It was clear that small differences were found in the SDS-PAGE analysis of wheat endosperm proteins among the studied genotypes. The defense isozymes [Peroxidase (Px), Polyphenol oxidase (PPO) and Malate dehydrogenase (Mdh)] were detected and their results concluded a prominent role, either directly or indirectly in stress responses. High polymorphisms were observed in most genotypes forMdh isozymes at Siwa habitat, meanwhile Px isozymes were decreased. Misr-1 genotype attained a higher level of Px isozymes under stress habitat as a trait to tolerance response. The changes of PPO isozymes showed a good correlation with the abiotic stress tolerance of wheat genotypes. Thus, PPO increased in Nesr, S8/17, ACSAD-2, ACSAD-3 and NBL genotypes under stress condition (Siwa habitat).
 
Keywords
Wheat genotypes; isozymes Siwa Oasis and Ashmon habitats; Protein profiling; peroxidase; Polyphenol oxidase and Malate dehydrogenase
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