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El-Shouny, W., Osman, M., Talat, R., El-Zahaby, H. (2017). The Immunoprotective Efficacy of Exopolysaccharides Produced from Different Strains of Pseudomonas syringae against Human Pathogenic Pseudomonas aeruginosa . Egyptian Journal of Botany, 57(7th International Conf.), 197-204. doi: 10.21608/ejbo.2017.971.1082
Wagih El-Shouny; Mohamed EH Osman; Ragdah Talat; Heba El-Zahaby. "The Immunoprotective Efficacy of Exopolysaccharides Produced from Different Strains of Pseudomonas syringae against Human Pathogenic Pseudomonas aeruginosa ". Egyptian Journal of Botany, 57, 7th International Conf., 2017, 197-204. doi: 10.21608/ejbo.2017.971.1082
El-Shouny, W., Osman, M., Talat, R., El-Zahaby, H. (2017). 'The Immunoprotective Efficacy of Exopolysaccharides Produced from Different Strains of Pseudomonas syringae against Human Pathogenic Pseudomonas aeruginosa ', Egyptian Journal of Botany, 57(7th International Conf.), pp. 197-204. doi: 10.21608/ejbo.2017.971.1082
El-Shouny, W., Osman, M., Talat, R., El-Zahaby, H. The Immunoprotective Efficacy of Exopolysaccharides Produced from Different Strains of Pseudomonas syringae against Human Pathogenic Pseudomonas aeruginosa . Egyptian Journal of Botany, 2017; 57(7th International Conf.): 197-204. doi: 10.21608/ejbo.2017.971.1082

The Immunoprotective Efficacy of Exopolysaccharides Produced from Different Strains of Pseudomonas syringae against Human Pathogenic Pseudomonas aeruginosa

Article 27, Volume 57, 7th International Conf., October 2017, Page 197-204  XML PDF (764.71 K)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2017.971.1082
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Authors
Wagih El-Shouny email 1; Mohamed EH Osman2; Ragdah Talat3; Heba El-Zahaby1
1microbiology, science, tanta univercity
2Tanta University, Egypt
3microbiology, medecine, tanta university
Abstract
EXOPOLYSACCHARIDES (EPS) produced from Pseudomonas syringae pv. tomato, P. syringae davson 973 and P. syringae pv. coriandricola 908 were studied. Mice immunized (i.p.) with bacterial EPS preparations from three strains (divided into 3 days) and in the fifth day, challenged (s.c.) with 1 ml of human pathogen P. aeruginosa 3 to each mouse, all groups of mice showed an increase in total leukocyte counts as well as neutrophil and monocyte numbers if compared with saline control group. Studing the active immunization by purified bacterial EPS on the counts of P. aeruginosa in lungs, spleens and livers of challenged mice revealed the protection of organs against bacteremia. Thus, the immunoprotective characters of the three purified EPSs could be concluded and recommended for prophylactic applications.
Keywords
Exopolysaccharides; P. aeruginosa ; Mice; Immunization; Bacteremia
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