Document Type : Regular issue (Original Article)
Authors
1
Botany and Microbiology department, faculty of science, Cairo university, Giza, Egypt
2
Professor of Microbiology, Botany and Microbiology Department, Faculty of Science, Cairo University, Egypt
3
Assistant Professor of Microbiology, Botany and Microbiology Department, Faculty of Science, Cairo University, Egypt
4
Assistant Professor at Egyptian atomic energy authority, Nuclear Research Center, Nuclear physics department
5
Head of Agricultural by product Evaluation Lab, Regional center for food and feed, Agricultural Research center, Giza, Egypt
Abstract
Oyster mushroom (Pleurotus ostreatus) has high nutritional value, containing biologically active compounds and therapeutic ingredients. Fruiting bodies of P. ostreatus were irradiated with different irradiation dosages of gamma rays of 0.25, 0.5, 1.0, and 2.0 kGy and then extracted using diethyl ether, ethyl acetate, methyl alcohol, ethyl alcohol, chloroform, and water. The antibacterial properties of the extract were evaluated against a strain of Gram-positive bacteria, Staphylococcus aureus, and Gram-negative bacteria, including Pseudomonas aeruginosa, Klebsiella spp., and Escherichia coli, using the disk diffusion assay. All the tested bacteria were sensitive to diethyl ether extract except P. aeruginosa, which was resistant. However, all tested bacteria were resistant to the other extracts. The most effective diethyl ether extract was selected for examination using gas chromatography-mass spectrometry (GC-MS). GC-MS examination revealed the existence of pharmaceutical substances such as cholest-5-en-3-ol, 1,2-benzenedicarboxylic acid, and oleic acid. GC-MS also displayed the synthesis of new chemical compounds, such as methyl 10,12-pentacosadiynoate, as well as the degradation of some chemical compounds that were detected, such as 9-octadecenoic acid (Z), methyl ester, and (Z)-9-octadecenamide was observed.
Keywords
)
View on SCiNiTO