The significance of the endophyte Pseudomonas aeruginosa in enhancing wheat seedlings' responses to salinity stress

Document Type : Regular issue (Original Article)

Authors

1 Department of botany,Fayoum university,Egypt

2 Botant Department, Faculty of Science, Fayoum university

3 Botany Department, Faculty of Science, Fayoum University, 63514 Fayoum, Egypt

Abstract

Salinity is a potentially dangerous environmental state that has a substantial impact on plant productivity. Many adaptation strategies are required in order to withstand salt stress. These remedies, meanwhile, seem expensive and time-consuming. Recent research has revealed that plant growth promoting endophytes (PGPEs), which can strengthen their symbiotic relationship with their host to increase the host plant's capacity to withstand salt. Our study's primary objective is to increase plant salt tolerance by utilizing endophytic bacterial species. One isolate of the eight endophytic bacterial species that were isolated from the roots, stems, and leaves of the Zilla spinosa plant exhibited positive results for ACC deaminase activity and the capacity to use 1-aminocyclopropane-1-carboxylic acid (ACC) as the sole source of nitrogen. Using 16S rRNA gene sequencing, the bacterial isolate was identified as Pseudomonas aeruginosa, and it received the genbank accession number PP819377. Our findings showed that Pseudomonas aeruginosa is a valuable biological plant growth promoter that enhanced wheat plant salt tolerance under 50, 100, and 150 mM NaCl levels when compared to the non-inoculated controls. An increase in plant growth parameters and the responses of enzymatic and non-enzymatic constituents of ascorbate-glutathione redox cycle were demonstrated. The co-inoculation of the chosen endophytic bacterial strain, which was successfully isolated from halotolerant plants, greatly reduced the negative impacts of salt stress and enhanced plant development and biomass output, according to our findings.

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