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Sabry, A., Shindia, A., El-Badry, W. (2025). Cholesterol oxidase; Sources, biochemical properties and therapeutic applications. Egyptian Journal of Botany, 65(3), 537-544. doi: 10.21608/ejbo.2025.358992.3183
Ashraf Sabry; Ahmed A. Shindia; Walla M. El-Badry. "Cholesterol oxidase; Sources, biochemical properties and therapeutic applications". Egyptian Journal of Botany, 65, 3, 2025, 537-544. doi: 10.21608/ejbo.2025.358992.3183
Sabry, A., Shindia, A., El-Badry, W. (2025). 'Cholesterol oxidase; Sources, biochemical properties and therapeutic applications', Egyptian Journal of Botany, 65(3), pp. 537-544. doi: 10.21608/ejbo.2025.358992.3183
Sabry, A., Shindia, A., El-Badry, W. Cholesterol oxidase; Sources, biochemical properties and therapeutic applications. Egyptian Journal of Botany, 2025; 65(3): 537-544. doi: 10.21608/ejbo.2025.358992.3183

Cholesterol oxidase; Sources, biochemical properties and therapeutic applications

Article 44, Volume 65, Issue 3, July 2025, Page 537-544  XML PDF (1.27 MB)
Document Type: Regular Issue (Review)
DOI: 10.21608/ejbo.2025.358992.3183
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Authors
Ashraf Sabry email 1; Ahmed A. Shindia2; Walla M. El-Badry2
1Zagazig Univ., Mycology & Molecular Biology
2Zagazig University
Abstract
Cholesterol oxidase is a profound enzyme that recently gained much attention, especially for its numerous therapeutic and biotechnological applications, as a rapid and sensitive assay for cholesterol estimation in various clinical samples, by catalyzing the conversation of cholesterol into cholest-4-en-3-one and reducing oxygen to hydrogen peroxide. The immobilized enzyme has been commonly used as an electrode in the colorimetric biosensors of cholesterol estimation, with a high sensitivity and selectivity for arteriosclerosis diagnosis, preparation of the ketosteroids from the corresponding ones, and steroids analysis in food samples. This enzyme was partially purified and characterized from various microbial sources. Nevertheless, the availability, structural stability, and catalytic efficiency of the completely purified cholesterol oxidase remain the major challenges. So, the objective of this review was to give an insight about the different sources, biochemical and catalytic properties of cholesterol oxidase from the literature study, in addition to the possible approaches to increase its reusability and operative stability via different immobilization techniques.
Keywords
Cholesterol Oxidase; Bacterial sources; Biochemical properties; Catalytic efficiency
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