THE PRODUCTION of cellulase from some local fungal isolates ……..was studied. The results showed that Aspergillus flavus produced the highest cellulase activity (3.017 U/ml) under static condition. It also showed that the best carbon source for cellulase production was filter papers (7.0932 U/ml), and onion scales (6.396 U/ml) at a concentration 30 g/l medium. The Plackett-Burman design was studied for cellulase production from A. flavus showed that the optimizing medium achieved by the statistical design increased cellulase activity (11.699 U/ml) which approximately 2.3 times than that obtained from the basal medium. In addition, A. flavus biomass was entrapped in different gel materials, and the best one for producing cellulase was agar (8.22 U/ml), however, this activity was lower than that in free cultures. Moreover, A. flavus biomass was adsorbed on different porous materials, and the cultures adsorbed on luffa pulp exerted the highest cellulase activity (17.78 U/ml) which was higher than that produced by free cultures. By reusing the adsorbed A. flavus biomass on luffa pulp in seven successive times, the 4th cycle gave the highest cellulase activity (34.17 U/ml). It was concluded that A. flavus is a feasible candidate for the production of cellulase activity which can be applied in many industrial fields. The results also indicate the possibility of using different agro-wastes like onion scales as a sole carbon source for production of cellulase by A. flavus.
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