Abdel- Sater, M., Abdel- Hafez, S., Hussein, N., al-amery, E. (2017). Fungi Associated with Maize and Sorghum Grains and their Potential for Amylase and Aflatoxins Production. Egyptian Journal of Botany, 57(1), 119-137. doi: 10.21608/ejbo.2017.296.1008
Mohamed Abdel- Sater; Sobhy Abdel- Hafez; Nemmat Hussein; Eshraq al-amery. "Fungi Associated with Maize and Sorghum Grains and their Potential for Amylase and Aflatoxins Production". Egyptian Journal of Botany, 57, 1, 2017, 119-137. doi: 10.21608/ejbo.2017.296.1008
Abdel- Sater, M., Abdel- Hafez, S., Hussein, N., al-amery, E. (2017). 'Fungi Associated with Maize and Sorghum Grains and their Potential for Amylase and Aflatoxins Production', Egyptian Journal of Botany, 57(1), pp. 119-137. doi: 10.21608/ejbo.2017.296.1008
Abdel- Sater, M., Abdel- Hafez, S., Hussein, N., al-amery, E. Fungi Associated with Maize and Sorghum Grains and their Potential for Amylase and Aflatoxins Production. Egyptian Journal of Botany, 2017; 57(1): 119-137. doi: 10.21608/ejbo.2017.296.1008
Fungi Associated with Maize and Sorghum Grains and their Potential for Amylase and Aflatoxins Production
1Botany and Microbiology Department Faculty of Science Assiut University Assiut, Egypt
2Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, Egypt
3Assiut University, Faculty of Science, Botany & Microbiology Department
Abstract
USING direct-plating technique, thirty-nine species belonging to 16 fungal genera were isolated from maize and sorghum grain samples (20 samples each) on Czap ek’s agar (Cz) and Czap ek’s supplemented with 40% sucrose agar (Cz40S) media at 28ºC. Widest spectrum of genera and species were recorded on sorghum (16 genera and 35 species) compared with maize grains (8 and 19). The highest total counts (139 CFUs/ 100 grains) and the number of genera (14) and species (27) were identified from sorghum grains on Cz medium. The most common fungi on the grains tested were Aspergillus flavus, A. niger, Eurotium amstelodami, E. rubrum, E. repens, Fusarium verticillioides and Rhizopus stolonifer. Among 129 isolates screened for their abilities to produce amylase enzyme, 102 isolates could produce this enzyme, of which A. terreus exhibited the highest production (EI=1.73). HPLC analysis revealed that out of 6 strains of A. flavus tested, strain No. AUMC 11311 showed the highest production of aflatoxin B1 and B2, while the highest value of aflatoxin G2 was produced by strain No. AUMC 11317. It could be concluded that fungi growing on grains and their abilities for producing enzymes and aflatoxins might cause deterioration and spoilage of these grains.
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