Aly, H., Saber, N., Mohamed, A. (2018). Effect of Sodium Nitro-Prusside (SNP) Preatreatment on Ammonia Assimilating Enzymes of Salt Stressed Tomato Leaves ( Lycopersicon esculentum ). Egyptian Journal of Botany, 58(3), 491-500. doi: 10.21608/ejbo.2018.3064.1160
Hala Ezzat Mouhamed Aly; Nabil Saber; Amany Gaber Mohamed. "Effect of Sodium Nitro-Prusside (SNP) Preatreatment on Ammonia Assimilating Enzymes of Salt Stressed Tomato Leaves ( Lycopersicon esculentum )". Egyptian Journal of Botany, 58, 3, 2018, 491-500. doi: 10.21608/ejbo.2018.3064.1160
Aly, H., Saber, N., Mohamed, A. (2018). 'Effect of Sodium Nitro-Prusside (SNP) Preatreatment on Ammonia Assimilating Enzymes of Salt Stressed Tomato Leaves ( Lycopersicon esculentum )', Egyptian Journal of Botany, 58(3), pp. 491-500. doi: 10.21608/ejbo.2018.3064.1160
Aly, H., Saber, N., Mohamed, A. Effect of Sodium Nitro-Prusside (SNP) Preatreatment on Ammonia Assimilating Enzymes of Salt Stressed Tomato Leaves ( Lycopersicon esculentum ). Egyptian Journal of Botany, 2018; 58(3): 491-500. doi: 10.21608/ejbo.2018.3064.1160
Effect of Sodium Nitro-Prusside (SNP) Preatreatment on Ammonia Assimilating Enzymes of Salt Stressed Tomato Leaves ( Lycopersicon esculentum )
1Botany Department, Faculty of Science, Alexandria University, Alexandria, Egypt
2Biological and Geological Department, Faculty of Education, Alexandria University, Alexandria, Egypt
Abstract
THE EFFECTS of treatment with 100mM NaCl on photosynthetic activity, protein and proline contents , activities of key nitrogen metabolism enzymes, nitrate reductase (NR) and nitrite reductase (NiR), ammonia assimilating enzymes: Glutamine synthetase (GS), glutamate synthase (GOGAT) and glutamate dehydrogenase (NADH-GDH) activities and finally ammonia and nitrate contents were investigated in 40-day old leaves of tomato plants. There was a decline in total protein (TP) and insoluble protein (InsP) fractions accompanied with a significant increase in proline and soluble protein contents in response to NaCl – stress. Under salinized conditions, there was a significant inhibition in all tested leaf gas exchange parameters, stomatal conductance (gs), internal CO2 concentration (Ci) and hence, CO2 assimilation (A). Also, a significant inhibition of NR & NiR enzymes and a strongly decrease in nitrate content were observed. In contrast, ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities were obviously increased in NaCl – salinized tomato leaves, this accompanied with a significant increase in ammonia content. Soaking tomato seeds in 10μM sodium nitroprusside (SNP) for 8h were elevated-to some extent- all the studied parameters and there was an improvement in TP, proline, all gas exchange parameters, NR & NiR activities and nitrate content. While ammonia assimilating enzymes (GS, GOGAT, NADH-GDH and NAD-GDH) activities and ammonia content were significantly decreased compared to NaCl - salinized tomato leaves.
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