Isolation and Identification of Potent Probiotics with High Lead Removal Capability

Document Type : Regular issue (Original Article)

Authors

1 Microbiology Department, National Center of Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt

2 Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Abstract

Probiotics play an effective and significant role in human health. The aim of this study was to isolate potential probiotics from Egyptian sources. Among the 14 different bacterial isolates recovered from 8 different sources on Man-Rogosa-Sharp (MRS) agar medium, 5 isolates exhibited tolerance to pH 3 and survived at bile concentration of 0.3% for 3h. The selected isolates were resistant to amikacin, vancomycin, ciprofloxacin and bacitracin. In addition, they showed high antibacterial activity against 7 pathogens. Hydrophobicity using
xylene and toluene showed high hydrophobic property for M isolate with toluene and xylene (80.43±0.95% and 78.2±0.73%, respectively). The potent isolate was identified by 16S rRNA gene as Lactobacillus plantarum strain M (KY508300). This strain was able to remove 71.28 ± 0.4 % of lead (1mg/ml) after 5min. The probiotic strain M showed count stability in yoghurt up to 21 days. The characteristic features of L. plantarum strain M as potent probiotics entitled it to be used in industrial and environmental applications.

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