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Mahmoud, O., Abdel_Hadi, S. (2022). Extraction and Purification of Lovastatin from the Edible Mushroom Laetiporus sulphureus and its Antioxidant Activity. Egyptian Journal of Botany, 62(1), 169-175. doi: 10.21608/ejbo.2021.82715.1722
Othman A. Mahmoud; Shimal Y. Abdel_Hadi. "Extraction and Purification of Lovastatin from the Edible Mushroom Laetiporus sulphureus and its Antioxidant Activity". Egyptian Journal of Botany, 62, 1, 2022, 169-175. doi: 10.21608/ejbo.2021.82715.1722
Mahmoud, O., Abdel_Hadi, S. (2022). 'Extraction and Purification of Lovastatin from the Edible Mushroom Laetiporus sulphureus and its Antioxidant Activity', Egyptian Journal of Botany, 62(1), pp. 169-175. doi: 10.21608/ejbo.2021.82715.1722
Mahmoud, O., Abdel_Hadi, S. Extraction and Purification of Lovastatin from the Edible Mushroom Laetiporus sulphureus and its Antioxidant Activity. Egyptian Journal of Botany, 2022; 62(1): 169-175. doi: 10.21608/ejbo.2021.82715.1722

Extraction and Purification of Lovastatin from the Edible Mushroom Laetiporus sulphureus and its Antioxidant Activity

Article 14, Volume 62, Issue 1, January 2022, Page 169-175  XML PDF (1.7 MB)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2021.82715.1722
Cited by Scopus (6)
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Authors
Othman A. Mahmoud email ; Shimal Y. Abdel_Hadi
Department of Biology, College of Education for Pure Sciences, Mosul University, Mosul, Iraq
Abstract
LOVASTATIN is a cholesterol-lowering drug produced by the secondary metabolism of different fungi. In the current study, lovastatin was extracted from the basidiomycetes fungus Laetiporus sulphureus by submerged fermentation using an organic solvent ethyl acetate. Initial detection of lovastatin production in the crude extract was carried out using agar well diffusion method and was found to have inhibitory activity against yeast Candida albicans. Thin-layer chromatography (TLC) was used to detected lovastatin, and a distinct spot with an RF value of 0.55 appeared similar to standard Lovastatin. A single peak was also featured with high-performance liquid chromatography (HPLC) with a retention time of 14.61 minutes to see the purity of Lovastatin. The result of the detection of active groups in lovastatin purified by the infrared spectrum indicated the presence of amide and carboxyl groups. The purified lovastatin showed antioxidant efficacy with the DPPH test, with the inhibitory effect reaching 75% at a concentration of 100μg/mL compared with the inhibitory effect of standard antioxidant, ascorbic acid, which reached 85%.
Keywords
DPPH assay; Edible mushroom; HPLC analysis; Laetiporus sulphureus; Lovastatin; TLC
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