(2016). Response of Moringa oleifera Callus and Plantlets to Mannitol-induced Drought Stress.. Egyptian Journal of Botany, 56(3), 647-668. doi: 10.21608/ejbo.2016.2723
. "Response of Moringa oleifera Callus and Plantlets to Mannitol-induced Drought Stress.". Egyptian Journal of Botany, 56, 3, 2016, 647-668. doi: 10.21608/ejbo.2016.2723
(2016). 'Response of Moringa oleifera Callus and Plantlets to Mannitol-induced Drought Stress.', Egyptian Journal of Botany, 56(3), pp. 647-668. doi: 10.21608/ejbo.2016.2723
Response of Moringa oleifera Callus and Plantlets to Mannitol-induced Drought Stress.. Egyptian Journal of Botany, 2016; 56(3): 647-668. doi: 10.21608/ejbo.2016.2723
Response of Moringa oleifera Callus and Plantlets to Mannitol-induced Drought Stress.
IN THIS work, we generated callus and micropropagated plants fromMoringa oleifera to evaluate their response to drought stress induced by different concentrations of mannitol. Mannitol-induced drought stress caused a reduction shoot and root length as well as fresh and dry weights of shoots and roots in micropropagated plants. In addition, chlorophyll (a and b) and carotenoids contents were reduced. Such a reduction was concomitant with increases in soluble sugars, proline, malondialdehyde (MDA), ascorbic acid (ASA), reduced glutathione (GSH) and total phenols content in both callus and micropropagated plants. Moreover, the activities of catalase (CAT), superoxide dismutase (SOD), polyphenol oxidase (PPO) increased in micropropagated plants parallel with decreases in peroxidase (POX), ascorbate peroxidase (APX) and ascorbate oxidase (ASO) activities. On the other hand, the activities of CAT, SOD, POX, PPO, ASO increased in callus coupled with a decrease in APX. Our data indicated that micropropagated plants are more sensitive to drought stress, compared to callus as evidenced by greater accumulation of MDA while callus accumulated more soluble sugar, proline, GSH and total phenols than micropropagated plants.
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