Characterization of a Novel Specific Mouse Monoclonal Antibody Targeted to Envelope Protein E1/E2 of Hepatitis C Virus

doi:10.21608/ejbo.2016.2734

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	Characterization of a Novel Specific Mouse Monoclonal Antibody Targeted to Envelope Protein E1/E2 of Hepatitis C Virus
Article 13, Volume 56, Issue 3, September 2016, Page 669-677 PDF (378.54 K)
Document Type: Regular issue (Original Article)
DOI: 10.21608/ejbo.2016.2734
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Abstract
HEPATITIS C virus has been proven to be a major disease over …….. the world and widely present in Egypt. The aim of this study was to propagate, characterize and test the reactivity of the hybridoma cell line (7G9) which produces a novel mouse monoclonal antibody (MoAb) targeting HCV E1/E2 envelope protein. The propagation of this hybridoma cell line (7G9) was generated using standard hybridoma technology. Isotyping of the generated mouse antibody was done by commercial ELISA kit. The reactivity of (7G9) was assessed by ELISA plates coated by HCV E1/E2 derived from cell lysate transfected by plasmid expressed HCV E1/E2. The generated monoclonal antibody (7G9) was found to be an IgM antibody with a kappa light chain. ELISA showed that (7G9) reacted with cell lysate expressed HCV E1/E2 and (7G9) presented no cross-reactivity with different antigens such as Brucella abortus, Salmonella typhi and Hepatitis B Virus (HBV) antigens. ELISA revealed high reactivity of (7G9) with HCV E1 (a.a. 315-323) antigen. Thus, the mouse monoclonal antibody (7G9) can be used for immunodiagnosis of HCV infection by detection of HCV E1/E2 antigens.
Keywords
Hepatitis C virus; Envelope Protein E1/E2; Hybridoma cell line (7G9)


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