Alphonse, M., Thiagarajan, K., Navapara, R., Doshi, R., Sabade, N., Abdi, G., Singh, S., Fulzele, D., Pillay, M., Ramamoorthy, S., Chandrasekaran, R. (2024). Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus. Egyptian Journal of Botany, 64(4), 120-126. doi: 10.21608/ejbo.2024.240513.2582
Mariadoss Alphonse; Kalaivani Thiagarajan; Rahda Navapara; Richa Doshi; Nidhi Sabade; Gholamreza Abdi; Sudhir Singh; Devanand P Fulzele; Michael Pillay; Siva Ramamoorthy; Rajasekaran Chandrasekaran. "Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus". Egyptian Journal of Botany, 64, 4, 2024, 120-126. doi: 10.21608/ejbo.2024.240513.2582
Alphonse, M., Thiagarajan, K., Navapara, R., Doshi, R., Sabade, N., Abdi, G., Singh, S., Fulzele, D., Pillay, M., Ramamoorthy, S., Chandrasekaran, R. (2024). 'Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus', Egyptian Journal of Botany, 64(4), pp. 120-126. doi: 10.21608/ejbo.2024.240513.2582
Alphonse, M., Thiagarajan, K., Navapara, R., Doshi, R., Sabade, N., Abdi, G., Singh, S., Fulzele, D., Pillay, M., Ramamoorthy, S., Chandrasekaran, R. Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus. Egyptian Journal of Botany, 2024; 64(4): 120-126. doi: 10.21608/ejbo.2024.240513.2582
Elicitation of gentiopicroside in Gentiana kurroo Royle shoot cultures with endophytic fungus
21Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore – 632014, TamilNadu, India
3Department of Biotechnology, Persian Gulf Research Institute, Persian Gulf University, Bushehr, 75169, Iran
4Plant Biotechnology and Secondary Metabolites Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India Homi Bhabha National Institute, Anushaktinagar, Mumbai, 400094, India
5Plant Biotechnology and Secondary Metabolites Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, 400 085, India
6Department of Life and Consumer Sciences, College of Agriculture and Environmental Sciences, University of South Africa, Florida Campus, Johannesburg, South Africa
7School of Bio Sciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India
Abstract
Gentiopicroside (GPD) is a major secoiridoid glucoside in the herbs of the Gentianaceae family, predominantly in the genus Gentiana. Gentiana kurroo Royle, a native of the Indian Himalayas is rich in GPD in the roots and shoots. It is used extensively for treating various diseases in Ayurvedic medicine. This herb grows above 1600–3500 Masl and is endemic to the Himalayan region and extensively harvested from wild sources for its immense medicinal properties. In this study, in vitro shoot cultures of G. kurroo were stimulated with Trichoderma viride, Aspergillus niger, Fusarium oxysporum, and an endophytic fungal cell wall extract for enhanced production of GPD. The results showed that treatment with the endophytic fungal extract produced a much higher concentration of GPD (30.4 mg/g DW) after 48 hrs of treatment compared with other treatments. The endophytic fungus was identified as Parengyodontium album. This study provides a simple and efficient method for in vitro elicitation of GPD.
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