Khalifa, N., Shehata, M., Sakran, A., Shehata, M., Abdelsattar, M. (2017). Differential ُExpression of RuBisCO large Subunit and its Assembly in Intact Chloroplasts of Pisum sativum L. Treated with NaCl and Jasmonic Acid. Egyptian Journal of Botany, 57(3), 525-537. doi: 10.21608/ejbo.2017.1050.1092
Noha Khalifa; Mohamed Shehata; Ayat Sakran; Maher Shehata; Mohamed Abdelsattar. "Differential ُExpression of RuBisCO large Subunit and its Assembly in Intact Chloroplasts of Pisum sativum L. Treated with NaCl and Jasmonic Acid". Egyptian Journal of Botany, 57, 3, 2017, 525-537. doi: 10.21608/ejbo.2017.1050.1092
Khalifa, N., Shehata, M., Sakran, A., Shehata, M., Abdelsattar, M. (2017). 'Differential ُExpression of RuBisCO large Subunit and its Assembly in Intact Chloroplasts of Pisum sativum L. Treated with NaCl and Jasmonic Acid', Egyptian Journal of Botany, 57(3), pp. 525-537. doi: 10.21608/ejbo.2017.1050.1092
Khalifa, N., Shehata, M., Sakran, A., Shehata, M., Abdelsattar, M. Differential ُExpression of RuBisCO large Subunit and its Assembly in Intact Chloroplasts of Pisum sativum L. Treated with NaCl and Jasmonic Acid. Egyptian Journal of Botany, 2017; 57(3): 525-537. doi: 10.21608/ejbo.2017.1050.1092
Differential ُExpression of RuBisCO large Subunit and its Assembly in Intact Chloroplasts of Pisum sativum L. Treated with NaCl and Jasmonic Acid
1Botany Department, Faculty of Science, Ain Shams University, Cairo, Egypt
2Plant Molecular Biology Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Cairo, Egypt
Abstract
THIS STUDY aimed to detect the effect of stress conditions imposed by different treatments of NaCl and jasmonic acid (JA) on the expression pattern and assembly of RuBisCO large subunit (RbcL) and RuBisCO enzyme complex using SDS-PAGE and HDN-PAGE techniques, respectively. Both Total Soluble Proteins (TSPs) and Chloroplast Proteins (ChPs) were variably affected by each treatment. Notably, a unique protein band was detected in the seedlings treated with 150 mM NaCl for 5 days. Surprisingly, this band was not detected under any other salt treatment. Using nano HPLC-ESI-MS/MS MALDI-TOF protein sequencing, this band was identified as downshifted RbcL at approximately 50 kDa in addition to the original one was separated at approximately 55 kDa on SDS-PAGE. The expression of RbcL protein decreased in a dose dependent manner in the plants treated with JA for one and five days. All treatments did not affect the RuBisCO complex assembly at approximately 660 kDa, but triggered stress-induced ChPs at approximately 440 kDa and 1 MDa concomitant with NaCl treatments. RbcL band isolated from intact chloroplast was associated with Coupling Factor (CF1) beta subunit of ATP synthase as revealed by MALDI-TOF protein sequencing analysis. Possible interpretations of these findings have been further discussed in this study.
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