Fadl, F., Shanab, S., Shalaby, E. (2025). Antioxidant and anticancer potential of successive extracts and column fractions of three microalgae were measured for optimum conditions. Egyptian Journal of Botany, 65(2), 47-65. doi: 10.21608/ejbo.2024.287638.2892
Fatma El Zahraa Fadl; Sanaa Mahmoud Shanab; Emad Shalaby. "Antioxidant and anticancer potential of successive extracts and column fractions of three microalgae were measured for optimum conditions". Egyptian Journal of Botany, 65, 2, 2025, 47-65. doi: 10.21608/ejbo.2024.287638.2892
Fadl, F., Shanab, S., Shalaby, E. (2025). 'Antioxidant and anticancer potential of successive extracts and column fractions of three microalgae were measured for optimum conditions', Egyptian Journal of Botany, 65(2), pp. 47-65. doi: 10.21608/ejbo.2024.287638.2892
Fadl, F., Shanab, S., Shalaby, E. Antioxidant and anticancer potential of successive extracts and column fractions of three microalgae were measured for optimum conditions. Egyptian Journal of Botany, 2025; 65(2): 47-65. doi: 10.21608/ejbo.2024.287638.2892
Antioxidant and anticancer potential of successive extracts and column fractions of three microalgae were measured for optimum conditions
1Botany &Microbiology department,Faculty of Science,Cairo university
2Botany and Microbiology department, Faculty of Science, Cairo University, Egypt
3Biochemistry, Agriculture, Cairo University
Abstract
Three green microalgal species ( Chlamydomonas reinhardtii , Dictyochloropsis splendida , Muriella sp.) were cultured under four stress culture conditions (nitrogen concentrations , light intensity , photoperiod , salinity). The aim of the present study is to evaluate the algal growth parameters , antioxidant and anticancer efficiencies of the stressed biomasses. Extraction was performed by hexane , ethyl acetate , methanol and produced 36 extracts (E1-E36 ). Antioxidant activity of the 36 crude extracts were performed by radical DPPH & non-radical KMnO4. E14 of D. splendida cultivated on 3g L nitrogen was the promising crude extract exhibited the greatest antioxidant activity by both methods. Fractioation of E14 was done by silica gel column chromatography produced 11 fractions (F1—F11) . Greater antioxidant activity of F5, F8 occurred compared to antioxidant standards (Ascorbic acid, BHT). High Performance Liquid Chromatography ( HPLC ) analyses of E14 , F5 , F8 were evaluated and 4 ,5 , 6 Phenolic compounds were produced respectively. Anticancer activity of E14, F5 , F8 as well as the mixture of F5+ F8 was determined against three cancer cell lines ( HepG2 , MCF7 & Pc3 ) against MTT assay. The obtained results showed that fractions and mixture of fractions exhibited greater activity and lower IC50 compared to their crude extract E14 .
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