Decolorization of Remazol Brilliant Blue Dye by Escherichia coli NG188 under Optimized Conditions

Document Type : Regular issue (Original Article)

Authors

1 Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt

2 Botany Department, Faculty of Science, Damanhour University, Damanhour, Egypt

Abstract

THE OBJECTIVE of this study was to degrade Remazol Brilliant Blue (RB) textile dye by using Escherichia coli NG188 isolated from the industrial wastewater effluent. The optimum RB degradation (%) was obtained at; 40°C, inoculum size 5%, and pH 8 under static condition. The influence of nutritional factors on dye degradation via Plackett-Burman statistical design was investigated. On the basis of the calculated t-values, P values and confidence level: glucose and dye concentration had confidence levels above 90% and hence were considered the significant parameters that influence dye degradation by Escherichia coli NG188. A verification experiment was carried out to evaluate the accuracy of the applied Plackett-Burman statistical design. The decolorization percentage showed a 1.24 fold increase in the degradation rate of the dye compared to the average of the basal condition results. The complete decolorization of the RB dye was reported using the Response Surface Methodology (RSM). The biodegradation of RB was confirmed by UV–visible spectroscopic and FTIR analysis.

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